A Novel Fluorescent Aptasensor Based on Dual-labeled DNA Nanostructure for Simultaneous Detection of Ochratoxin A and Aflatoxin B1

• Published in: Journal of fluorescence Vol. 33; no. 4; pp. 1641 - 1650
• Main Authors: Zhao, Luyang, Liang, Xiujun, Liu, Yong, Wei, Min, Jin, Huali
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.07.2023; Springer Nature B.V
• Subjects: Aflatoxin B1 - chemistry; Aflatoxins; Analytical Chemistry; Biochemistry; Biological and Medical Physics; Biomedical and Life Sciences; Biomedicine; Biophysics; Biotechnology; DNA - chemistry; Energy transfer; Fluorescence; Fluorescent Dyes - chemistry; Limit of Detection; Nanostructure; Nanostructures; Simultaneous detection; Dual-labeled DNA nanostructure; Aptasensor; DNA strand replacement reaction

Based on DNA strand replacement reaction and aptamer-specific recognition, a simple dual-labeled DNA nanostructure is designed for the simultaneous detection of Ochratoxin A (OTA) and aflatoxin B1 (AFB 1 ). C1 is labeled with Cy3 and Cy5, while C2 and C3 are labeled with BHQ2. The fluorescence inten...