A “Turn-on” Fluorescent Probe Based on Phenothiazine for Selectively Recognizing ClO− and its Practical Applications

• Published in: Journal of fluorescence Vol. 33; no. 6; pp. 2451 - 2459
• Main Authors: Liu, Qianling, Li, Xue, Xiao, Ming, Ai, Yin, Liu, Gang, Ding, Haichang, Pu, Shouzhi
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.11.2023; Springer Nature B.V
• Subjects: Analytical Chemistry; Beansprouts; Biochemistry; Biological and Medical Physics; Biomedical and Life Sciences; Biomedicine; Biophysics; Biotechnology; Fluorescent indicators; Sensitivity; Synthesis; Testing strips; Fluorescent probe; ClO; Phenothiazine
• ISSN: 1053-0509; 1573-4994
• DOI: 10.1007/s10895-023-03215-1

Hypochlorous acid (HClO), a highly reactive oxygen species, has important effects on human health. High selectivity and sensitivity remain challenges of fluorescent probes for detection of ClO − with a large Stokes shift. This work designed and synthesized a novel phenothiazine-based fluorescent probe TF which can detect ClO − by colorimetric and fluorescent dual signals. TF displayed turn-on fluorescence effect toward ClO − with high selectivity (≥ 28-folds) and sensitivity (LOD = 0.472 μM), fast response time (< 1 min) and large Stokes shift (150 nm) in PBS (pH = 7.4, 40% DMSO). Meanwhile, TF can visualize ClO − on the mung bean sprouts model and apply as testing strips for portable and rapid detecting ClO − by the naked eyes. Graphical Abstract A phenothiazine-based fluorescent probe with large Stokes shift was synthesized and its responding rapidly ability to detect ClO − was studied.