Stage-specific analysis of plasma protein profiles in ovarian cancer: Difference in-gel electrophoresis analysis of pooled clinical samples
Introduction: Ovarian cancer is the leading cause of death from gynecological cancer. Non-specific symptoms early in disease and the lack of specific biomarkers hinder early diagnosis. Multi-marker blood screening tests have shown promise for improving identification of early stage disease; however,...
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| Published in | Journal of carcinogenesis Vol. 12; no. 1; p. 10 |
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| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
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India
Medknow Publications
01.01.2013
Medknow Publications & Media Pvt Ltd |
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| Abstract | Introduction: Ovarian cancer is the leading cause of death from gynecological cancer. Non-specific symptoms early in disease and the lack of specific biomarkers hinder early diagnosis. Multi-marker blood screening tests have shown promise for improving identification of early stage disease; however, available tests lack sensitivity, and specificity. Materials and Methods: In this study, pooled deeply-depleted plasma from women with Stage 1, 2 or 3 ovarian cancer and healthy controls were used to compare the 2-dimensional gel electrophoresis (2-DE) protein profiles and identify potential novel markers of ovarian cancer progression. Results/Discussion: Stage-specific variation in biomarker expression was observed. For example, apolipoprotein A1 expression is relatively low in control and Stage 1, but shows a substantial increase in Stage 2 and 3, thus, potential of utility for disease confirmation rather than early detection. A better marker for early stage disease was tropomyosin 4 (TPM4). The expression of TPM4 increased by 2-fold in Stage 2 before returning to "normal" levels in Stage 3 disease. Multiple isoforms were also identified for some proteins and in some cases, displayed stage-specific expression. An interesting example was fibrinogen alpha, for which 8 isoforms were identified. Four displayed a moderate increase at Stage 1 and a substantial increase for Stages 2 and 3 while the other 4 showed only moderate increases. Conclusion: Herein is provided an improved summary of blood protein profiles for women with ovarian cancer stratified by stage. |
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| AbstractList | Ovarian cancer is the leading cause of death from gynecological cancer. Non-specific symptoms early in disease and the lack of specific biomarkers hinder early diagnosis. Multi-marker blood screening tests have shown promise for improving identification of early stage disease; however, available tests lack sensitivity, and specificity.
In this study, pooled deeply-depleted plasma from women with Stage 1, 2 or 3 ovarian cancer and healthy controls were used to compare the 2-dimensional gel electrophoresis (2-DE) protein profiles and identify potential novel markers of ovarian cancer progression.
Stage-specific variation in biomarker expression was observed. For example, apolipoprotein A1 expression is relatively low in control and Stage 1, but shows a substantial increase in Stage 2 and 3, thus, potential of utility for disease confirmation rather than early detection. A better marker for early stage disease was tropomyosin 4 (TPM4). The expression of TPM4 increased by 2-fold in Stage 2 before returning to "normal" levels in Stage 3 disease. Multiple isoforms were also identified for some proteins and in some cases, displayed stage-specific expression. An interesting example was fibrinogen alpha, for which 8 isoforms were identified. Four displayed a moderate increase at Stage 1 and a substantial increase for Stages 2 and 3 while the other 4 showed only moderate increases.
Herein is provided an improved summary of blood protein profiles for women with ovarian cancer stratified by stage. Introduction: Ovarian cancer is the leading cause of death from gynecological cancer. Non-specific symptoms early in disease and the lack of specific biomarkers hinder early diagnosis. Multi-marker blood screening tests have shown promise for improving identification of early stage disease; however, available tests lack sensitivity, and specificity. Materials and Methods: In this study, pooled deeply-depleted plasma from women with Stage 1, 2 or 3 ovarian cancer and healthy controls were used to compare the 2-dimensional gel electrophoresis (2-DE) protein profiles and identify potential novel markers of ovarian cancer progression. Results/Discussion: Stage-specific variation in biomarker expression was observed. For example, apolipoprotein A1 expression is relatively low in control and Stage 1, but shows a substantial increase in Stage 2 and 3, thus, potential of utility for disease confirmation rather than early detection. A better marker for early stage disease was tropomyosin 4 (TPM4). The expression of TPM4 increased by 2-fold in Stage 2 before returning to "normal" levels in Stage 3 disease. Multiple isoforms were also identified for some proteins and in some cases, displayed stage-specific expression. An interesting example was fibrinogen alpha, for which 8 isoforms were identified. Four displayed a moderate increase at Stage 1 and a substantial increase for Stages 2 and 3 while the other 4 showed only moderate increases. Conclusion: Herein is provided an improved summary of blood protein profiles for women with ovarian cancer stratified by stage. INTRODUCTIONOvarian cancer is the leading cause of death from gynecological cancer. Non-specific symptoms early in disease and the lack of specific biomarkers hinder early diagnosis. Multi-marker blood screening tests have shown promise for improving identification of early stage disease; however, available tests lack sensitivity, and specificity. MATERIALS AND METHODSIn this study, pooled deeply-depleted plasma from women with Stage 1, 2 or 3 ovarian cancer and healthy controls were used to compare the 2-dimensional gel electrophoresis (2-DE) protein profiles and identify potential novel markers of ovarian cancer progression. RESULTS/DISCUSSIONStage-specific variation in biomarker expression was observed. For example, apolipoprotein A1 expression is relatively low in control and Stage 1, but shows a substantial increase in Stage 2 and 3, thus, potential of utility for disease confirmation rather than early detection. A better marker for early stage disease was tropomyosin 4 (TPM4). The expression of TPM4 increased by 2-fold in Stage 2 before returning to "normal" levels in Stage 3 disease. Multiple isoforms were also identified for some proteins and in some cases, displayed stage-specific expression. An interesting example was fibrinogen alpha, for which 8 isoforms were identified. Four displayed a moderate increase at Stage 1 and a substantial increase for Stages 2 and 3 while the other 4 showed only moderate increases. CONCLUSIONHerein is provided an improved summary of blood protein profiles for women with ovarian cancer stratified by stage. |
| Author | Bailey, Mark Ayhan, Mustafa Shield-Artin, Kristy Reisman, Simone Rice, Gregory Oliva, Karen |
| AuthorAffiliation | 1 Zoetis, Veterinary Medicine Research and Development, Melbourne, Australia 2 Baker IDI Heart and Diabetes Institute, Genomics and Systems Biology, Melbourne, Australia 3 University of Queensland, Centre for Clinical Research, translational Research, Brisbane, Queensland, Australia |
| AuthorAffiliation_xml | – name: 1 Zoetis, Veterinary Medicine Research and Development, Melbourne, Australia – name: 2 Baker IDI Heart and Diabetes Institute, Genomics and Systems Biology, Melbourne, Australia – name: 3 University of Queensland, Centre for Clinical Research, translational Research, Brisbane, Queensland, Australia |
| Author_xml | – sequence: 1 givenname: Mark surname: Bailey fullname: Bailey, Mark organization: Zoetis, Veterinary Medicine Research and Development, Parkville, Victoria – sequence: 2 givenname: Kristy surname: Shield-Artin fullname: Shield-Artin, Kristy organization: Baker IDI Heart and Diabetes Institute, Genomics and Systems Biology, Melbourne – sequence: 3 givenname: Karen surname: Oliva fullname: Oliva, Karen organization: University of Queensland, Centre for Clinical Research, translational Research, Brisbane, Queensland – sequence: 4 givenname: Mustafa surname: Ayhan fullname: Ayhan, Mustafa organization: University of Queensland, Centre for Clinical Research, translational Research, Brisbane, Queensland – sequence: 5 givenname: Simone surname: Reisman fullname: Reisman, Simone organization: University of Queensland, Centre for Clinical Research, translational Research, Brisbane, Queensland – sequence: 6 givenname: Gregory surname: Rice fullname: Rice, Gregory organization: University of Queensland, Centre for Clinical Research, translational Research, Brisbane, Queensland |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/23858298$$D View this record in MEDLINE/PubMed |
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| Copyright | Copyright: © Bailey 2013 |
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| Keywords | protein depletion disease stage Difference in-gel electrophoresis ovarian cancer |
| Language | English |
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transition in human hepatocellular carcinoma year: 2010 ident: key-10.4103/1477-3163.114216-47 publication-title: BMC Cancer contributor: fullname: Choi – start-page: e11137 volume-title: Integrative proteomic analysis of serum and peritoneal fluids helps identify proteins that are up-regulated in serum of women with ovarian cancer year: 2010 ident: key-10.4103/1477-3163.114216-21 publication-title: PLoS One contributor: fullname: Amon – start-page: 865 volume-title: Carbonic anhydrase inhibitors and the management of cancer year: 2007 ident: key-10.4103/1477-3163.114216-41 publication-title: Curr Top Med Chem contributor: fullname: Pastorekova – start-page: 6018 volume-title: Prognostic value of prostate secretory protein of 94 amino acids and its binding protein after radical prostatectomy year: 2006 ident: key-10.4103/1477-3163.114216-34 publication-title: Clin Cancer Res contributor: fullname: Reeves – start-page: 80 volume-title: Prefractionation enhances loading capacity and identification of basic proteins from human breast cancer tissues year: 2011 ident: key-10.4103/1477-3163.114216-18 publication-title: Anal Biochem contributor: fullname: Semaan – start-page: 157 volume-title: Alpha-1-acid glycoprotein year: 2000 ident: key-10.4103/1477-3163.114216-20 publication-title: Biochim Biophys Acta contributor: fullname: Fournier – start-page: 160 volume-title: Reduced plasma levels of coagulation factors in relation to prostate cancer year: 2002 ident: key-10.4103/1477-3163.114216-32 publication-title: Prostate contributor: fullname: Beecken – start-page: 3678 volume-title: Abnormalities of complement and its components in patients with acute leukemia, Hodgkin′s disease, and sarcoma year: 1976 ident: key-10.4103/1477-3163.114216-31 publication-title: Cancer Res contributor: fullname: Lichtenfeld – start-page: 1659 volume-title: Evaluation of protein loading techniques and improved separation in OFFGEL isoelectric focusing year: 2011 ident: key-10.4103/1477-3163.114216-17 publication-title: Electrophoresis contributor: fullname: Keidel – start-page: 327 volume-title: A recipe for proteomics diagnostic test development: The OVA1 test, from biomarker discovery to FDA clearance year: 2010 ident: key-10.4103/1477-3163.114216-7 publication-title: Clin Chem contributor: fullname: Fung – start-page: 62 volume-title: Evaluation of midkine and anterior gradient 2 in a multimarker panel for the detection of ovarian cancer year: 2010 ident: key-10.4103/1477-3163.114216-9 publication-title: J Exp Clin Cancer Res contributor: fullname: Rice |
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| Snippet | Introduction: Ovarian cancer is the leading cause of death from gynecological cancer. Non-specific symptoms early in disease and the lack of specific... Ovarian cancer is the leading cause of death from gynecological cancer. Non-specific symptoms early in disease and the lack of specific biomarkers hinder early... INTRODUCTIONOvarian cancer is the leading cause of death from gynecological cancer. Non-specific symptoms early in disease and the lack of specific biomarkers... |
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| Title | Stage-specific analysis of plasma protein profiles in ovarian cancer: Difference in-gel electrophoresis analysis of pooled clinical samples |
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