In vitro maturation of ovine oocyte in a modified granulosa cells co-culture system and alpha-tocopherol supplementation: effects on nuclear maturation and cleavage

• Published in: Journal of animal science and technology Vol. 57; no. 1; p. 27
• Main Authors: Adeldust, Hamideh, Zeinoaldini, Saeed, Kohram, Hamid, Amiri Roudbar, Mahmoud, Daliri Joupari, Morteza
• Format: Journal Article
• Language: English
• Published: Korea (South) The Korean Society of Animal Science and Technology 2015; BioMed Central; 한국축산학회
• Subjects: 축산학; In vitro fertilization; Embryo; Granulosa cell; In vitro maturation

This study was designed to investigate the effects of α-tocopherol and granulosa cells monolayer on nuclear maturation and cleavage rates of ovine cumulus-oocyte complexes (COCs). The COCs (n = 2814) were matured in maturation medium supplemented with various concentration of α-tocopherol (0, 5, 10,...