Identification of an unknown glycoprotein from whole cell lysate using conA and mass spectrometry

• Published in: Biochemistry and molecular biology education Vol. 46; no. 4; pp. 373 - 381
• Main Authors: Yan, Jianghong, Hu, Shupeng, Xu, Xiudan, Zhao, Dezhang, Wu, Mingjun, Luo, Suxin, Xia, Yong
• Format: Journal Article
• Language: English
• Published: United States Wiley-Blackwell 01.07.2018; Wiley Subscription Services, Inc
• Subjects: Affinity chromatography; Allied Health Personnel; Animals; Biochemistry; Cell Extracts - chemistry; Cells, Cultured; Chromatography, Affinity; conA; Concanavalin A - chemistry; CURE; glycoprotein; Glycoproteins - analysis; Humans; Interferon; Laboratories; Laboratory Experiments; Learning; Lipopolysaccharides; Mass Spectrometry; Mass spectroscopy; Medical laboratories; Medical research; Medicine; Mice; Molecular Biology; RAW 264.7 Cells; Science Experiments; Science Instruction; Scientific imaging; Sepharose - chemistry; Spectroscopy; Student Attitudes; Student Surveys; Students; Teaching Methods; Undergraduate Students; Western blotting; conA; mass spectrometry; CURE; glycoprotein

Medical laboratory technology major was set up to meet rapid development of science and medical research technology in 2013. Students majoring in medical laboratory had learnt a lot of techniques distributed among different specialized courses. But, they did not understand why they had to learn thes...