Digestive enzyme activities in mudskipper Boleophthalmus pectinirostris and Chinese black sleeper Bostrichthys sinensis

• Published in: Chinese journal of oceanology and limnology Vol. 28; no. 4; pp. 756 - 761
• Main Author: 吴仁协 洪万树 张其永
• Format: Journal Article
• Language: English
• Published: Heidelberg SP Science Press 01.07.2010; Springer Nature B.V
• Subjects: Amylases; Bile; Boleophthalmus pectinirostris; Bostrichthys sinensis; Carnivores; Diet; Digestive system; Earth and Environmental Science; Earth Sciences; Enzymatic activity; Enzyme activity; Enzymes; Fish; Gastrointestinal tract; Herbivores; Intertidal environment; Intertidal zone; Intestine; Intestines; Marine; Marine biology; Oceanography; Protease; Stomach; α-Amylase; 中华乌塘鳢; 中华绒螯蟹; 冬虫夏草; 大弹涂鱼; 消化酶活性; 淀粉酶活性; 胃蛋白酶; 蛋白酶活性; protease; non-specific bile salt-activated lipase; α-amylase; feeding habit
• ISSN: 0254-4059; 2096-5508; 1993-5005; 2523-3521
• DOI: 10.1007/s00343-010-9111-5

The mudskipper Boleophthalmus pectinirostris and Chinese black sleeper Bostrichthys sinensis occupy the intertidal zone.However,both species have their own unique diet.The former is an herbivore and the latter is a carnivore.In order to reveal the relationship between digestive enzyme activities and diets in the two species,the activities of protease（P）,non-specific bile salt-activated lipase（BAL） and α-amylase（A） were determined in the stomach and intestine of adult mudskipper B.pectinirostris and Chinese black sleeper B.sinensis.The results showed that the activities of protease,BAL and α-amylase in the intestine of B.pectinirostris were significantly（P〈0.05） higher than those in the stomach.In B.sinensis,gastric protease activity was not different from the intestinal protease（P〉0.05）,while BAL and α-amylase activities of the intestine were significantly（P〈0.05） higher than those of the stomach.The activity of gastric protease in B.sinensis was significantly（P〈0.05） higher than that in B.pectinirostris,while the activities of intestinal protease were not different between the two fish species（P〉0.05）.BAL activities of the stomach and intestine in B.sinensis were significantly（P〈0.05） higher than those in B.pectinirostris,while α-amylase activities of the stomach and intestine in B.pectinirostris were significantly（P〈0.05） higher than those in B.sinensis.The ratios of P/BAL,A/P and A/BAL of the digestive tract in B.pectinirostris were 1.5,107.3 and 158.6,respectively; and those in B.sinensis were 0.2,1.6 and 0.2,respectively.It can be concluded that food digestion in the adult B.pectinirostris is mainly carried out in the intestine,whereas in the adult B.sinensis it is initiated in the stomach and finishes in the intestine.The activities of BAL and α-amylase in B.pectinirostris and B.sinensis are well correlated with their diets.However,a clear-cut correlation between protease activity and diets is not found in these two species.