Enhancement of therapeutic monoclonal antibody production in CHO cells using 30Kc6 gene

• Published in: Process biochemistry (1991) Vol. 45; no. 12; pp. 1852 - 1856
• Main Authors: Wang, Zesong, Park, Ju Hyun, Park, Hee Ho, Tan, Wensong, Park, Tai Hyun
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.12.2010
• Subjects: 30Kc19; 30Kc6; Apoptosis; Bombyx mori; Cascades; CHO; Culture; Genes; Mitochondrial membrane potential; Monoclonal antibodies; Monoclonal antibody; Productivity; Receptors; Recombinant; Viability; 30Kc19; 30Kc6; Bombyx mori; CHO; Monoclonal antibody; Apoptosis; Mitochondrial membrane potential

Over-expression of anti-apoptotic cloned-genes is a widely used strategy for inhibiting apoptosis in mammalian cell culture. In our previous study, we reported Bombyx mori 30K gene improved the production of recombinant proteins in Chinese hamster ovary (CHO) cells. In this study, we reengineered th...