Quantitative description of the interaction between folate and the folate-binding protein from cow's milk

• Published in: Biochemical journal Vol. 382; no. Pt 1; pp. 215 - 221
• Main Authors: Nixon, Peter F, Jones, Marc, Winzor, Donald J
• Format: Journal Article
• Language: English
• Published: England Portland Press Ltd 15.08.2004
• Subjects: Animals; Carrier Proteins - chemistry; Carrier Proteins - metabolism; Cattle; Folate Receptors, GPI-Anchored; Folic Acid - metabolism; Hydrogen-Ion Concentration; Milk; Milk Proteins - chemistry; Milk Proteins - metabolism; Molecular Weight; Multiprotein Complexes - chemistry; Protein Binding; Receptors, Cell Surface - chemistry; Receptors, Cell Surface - metabolism

A detailed study has been carried out on the dependence of folate binding on the concentration of FBP (folate-binding protein) at pH 5.0, conditions selected to prevent complications arising from the pre-existing self-association of the acceptor. In contrast with the mandatory requirement that rever...