In vitro cell stretching technology (IsoStretcher) as an approach to unravel Piezo1-mediated cardiac mechanotransduction

• Published in: Progress in biophysics and molecular biology Vol. 159; pp. 22 - 33
• Main Authors: Guo, Yang, Merten, Anna-Lena, Schöler, Ulrike, Yu, Ze-Yan, Cvetkovska, Jasmina, Fatkin, Diane, Feneley, Michael P., Martinac, Boris, Friedrich, Oliver
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.01.2021
• Subjects: Animals; Biosensing Techniques - instrumentation; Biosensing Techniques - methods; Calcium - metabolism; Cell Line; Cell stretching device; Cells, Cultured; Dimethylpolysiloxanes - metabolism; HL-1; Humans; Ion Channels - metabolism; Male; Mechanosensitive ion channel; Mechanotransduction; Mechanotransduction, Cellular - physiology; Mice; Mice, Inbred C57BL; Models, Biological; Myocardium - cytology; Myocardium - metabolism; Myocytes, Cardiac - metabolism; Piezo1; Polydimethylsiloxane (PDMS); Stress, Mechanical; HL-1; Mechanotransduction; Piezo1; Mechanosensitive ion channel; Cell stretching device; Polydimethylsiloxane (PDMS)

The transformation of electrical signals into mechanical action of the heart underlying blood circulation results in mechanical stimuli during active contraction or passive filling distention, which conversely modulate electrical signals. This feedback mechanism is known as cardiac mechano-electric...