Heat shock protein 70 overexpression does not attenuate atrophy in botulinum neurotoxin type A-treated skeletal muscle

• Published in: Journal of applied physiology (1985) Vol. 119; no. 1; pp. 83 - 92
• Main Authors: Houston, Fraser E, Hain, Brian A, Adams, Thomas J, Houston, Kati L, O'Keeffe, Roderic, Dodd, Stephen L
• Format: Journal Article
• Language: English
• Published: United States American Physiological Society 01.07.2015
• Subjects: Animals; Botulinum Toxins, Type A - toxicity; Cytotoxicity; Electroporation; Forkhead Transcription Factors - biosynthesis; Forkhead Transcription Factors - genetics; Gene Expression - drug effects; Heat shock proteins; Hindlimb - pathology; HSP70 Heat-Shock Proteins - biosynthesis; Male; Muscle Contraction - drug effects; Muscle, Skeletal - drug effects; Muscle, Skeletal - pathology; Muscular Atrophy - metabolism; Musculoskeletal system; Nerve Tissue Proteins - biosynthesis; Nerve Tissue Proteins - genetics; NF-kappa B - metabolism; Peptide Hydrolases - drug effects; Peptide Hydrolases - genetics; Peptide Hydrolases - metabolism; Plasmids - drug effects; Plasmids - genetics; Proteasome Endopeptidase Complex - metabolism; Protein expression; Protein folding; Rats; Rats, Sprague-Dawley; Rodents; Toxins; nuclear factor-κB; Foxo; NF-κB; chemical denervation; ubiquitin proteasome system; Forkhead box O; protein degradation

Botulinum neurotoxin type A (BoNT/A) is used clinically to induce therapeutic chemical denervation of spastically contracted skeletal muscles. However, BoNT/A administration can also cause atrophy. We sought to determine whether a major proteolytic pathway contributing to atrophy in multiple models...