Multiplex–microsphere–quantitative polymerase chain reaction: Nucleic acid amplification and detection on microspheres

• Published in: Analytical biochemistry Vol. 432; no. 1; pp. 23 - 30
• Main Authors: Liang, Fang, Lai, Richard, Arora, Neetika, Zhang, Kang Liang, Yeh, Che-Cheng, Barnett, Graeme R., Voigt, Paul, Corrie, Simon R., Barnard, Ross T.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.01.2013
• Subjects: detection limit; fluorescent dyes; Fluorescent Dyes - chemistry; gene amplification; genes; influenza; Limit of Detection; Luminex; Microsphere; Microspheres; Multiplex; Multiplex Polymerase Chain Reaction; Neisseria; Nucleic Acid Amplification Techniques; nucleic acids; oligonucleotides; Polymerase chain reaction; Quantitative; quantitative polymerase chain reaction; Real time; Polymerase chain reaction; Microsphere; Quantitative; Real time; Luminex; Multiplex

We report the development of a new system to monitor the amplification of nucleic acids on microspheres. This was realized by the design of (i) a “universal” oligonucleotide “tagged” polymerase chain reaction (PCR) forward primer, (ii) a sensor sequence complementary to the universal sequence on the...