Integrating a DNA barcoding project with an ecological survey：a case study on temperate intertidal polychaete communities in Qingdao,China
In this study,we integrated a DNA barcoding project with an ecological survey on intertidal polychaete communities and investigated the utility of CO1 gene sequence as a DNA barcode for the classification of the intertidal polychaetes.Using 16S rDNA as a complementary marker and combining morphologi...
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| Published in | Chinese journal of oceanology and limnology Vol. 28; no. 4; pp. 899 - 910 |
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| Main Author | |
| Format | Journal Article |
| Language | English |
| Published |
Heidelberg
SP Science Press
01.07.2010
Springer Nature B.V |
| Subjects | |
| Online Access | Get full text |
| ISSN | 0254-4059 2096-5508 1993-5005 2523-3521 |
| DOI | 10.1007/s00343-010-9131-1 |
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| Abstract | In this study,we integrated a DNA barcoding project with an ecological survey on intertidal polychaete communities and investigated the utility of CO1 gene sequence as a DNA barcode for the classification of the intertidal polychaetes.Using 16S rDNA as a complementary marker and combining morphological and ecological characterization,some of dominant and common polychaete species from Chinese coasts were assessed for their taxonomic status.We obtained 22 haplotype gene sequences of 13 taxa,including 10 CO1 sequences and 12 16S rDNA sequences.Based on intra-and inter-specific distances,we built phylogenetic trees using the neighbor-joining method.Our study suggested that the mitochondrial CO1 gene was a valid DNA barcoding marker for species identification in polychaetes,but other genes,such as 16S rDNA,could be used as a complementary genetic marker.For more accurate species identification and effective testing of species hypothesis,DNA barcoding should be incorporated with morphological,ecological,biogeographical,and phylogenetic information.The application of DNA barcoding and molecular identification in the ecological survey on the intertidal polychaete communities demonstrated the feasibility of integrating DNA taxonomy and ecology. |
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| AbstractList | In this study, we integrated a DNA barcoding project with an ecological survey on intertidal polychaete communities and investigated the utility of CO1 gene sequence as a DNA barcode for the classification of the intertidal polychaetes. Using 16S rDNA as a complementary marker and combining morphological and ecological characterization, some of dominant and common polychaete species from Chinese coasts were assessed for their taxonomic status. We obtained 22 haplotype gene sequences of 13 taxa, including 10 CO1 sequences and 12 16S rDNA sequences. Based on intra- and inter-specific distances, we built phylogenetic trees using the neighbor-joining method. Our study suggested that the mitochondrial CO1 gene was a valid DNA barcoding marker for species identification in polychaetes, but other genes, such as 16S rDNA, could be used as a complementary genetic marker. For more accurate species identification and effective testing of species hypothesis, DNA barcoding should be incorporated with morphological, ecological, biogeographical, and phylogenetic information. The application of DNA barcoding and molecular identification in the ecological survey on the intertidal polychaete communities demonstrated the feasibility of integrating DNA taxonomy and ecology. |
| Author | 周红 张志南 陈海燕 孙仁华 王慧 郭磊 潘海建 |
| AuthorAffiliation | College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China |
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| CitedBy_id | crossref_primary_10_3390_d14020083 crossref_primary_10_3897_zookeys_1015_54387 crossref_primary_10_1080_15627020_2024_2306421 crossref_primary_10_1111_1755_0998_12441 crossref_primary_10_3109_19401736_2014_984166 crossref_primary_10_11646_zootaxa_5529_2_2 crossref_primary_10_11646_zootaxa_5555_1_1 crossref_primary_10_1371_journal_pone_0117790 crossref_primary_10_1071_IS21084 crossref_primary_10_1007_s12526_021_01244_2 crossref_primary_10_1016_j_bse_2012_04_019 crossref_primary_10_7717_peerj_11847 crossref_primary_10_1007_s00343_021_1068_z crossref_primary_10_1007_s10452_019_09717_0 |
| Cites_doi | 10.1007/BF00393803 10.1016/S0166-0934(00)00255-X 10.1111/j.1471-8286.2007.01678.x 10.1016/j.tig.2007.02.001 10.1098/rspb.2002.2218 10.1371/journal.pbio.0020354 10.1016/j.ympev.2006.11.021 10.1093/nar/22.22.4673 10.1111/j.1463-6409.2006.00289.x 10.1098/rsbl.2003.0025 10.1080/17451000510018962 10.1093/molbev/msm092 10.1098/rstb.2005.1722 10.1007/s10152-005-0015-2 10.1073/pnas.0406166101 10.1111/j.1096-0031.2003.00008.x 10.1093/oxfordjournals.molbev.a003995 |
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| References | Antoniadou, Chintiroglou (CR1) 2006; 60 Ratnasingham, Hebert (CR20) 2007; 7 Schander, Willassen (CR21) 2005; 1 Wu, Wu, Qiu, Lu (CR27) 1997 Zhou (CR30) 2008 Clarke, Warwick (CR2) 2001 Thompson, Higgins, Gibson (CR25) 1994; 22 Tamura, Dudley, Nei, Kumar (CR24) 2007; 24 Wu, Sun, Yang (CR28) 1981 DeSalle, Egan, Siddall (CR4) 2005; 360 Folmer, Black, Hoeh, Lutz, Vrijenhoek (CR8) 1994; 3 Hajibabaei, Singer, Hebert, Hickey (CR9) 2007; 23 Hebert, Cywinska, Ball, deWaard (CR11) 2003; 270 Liao, Lin (CR15) 2006; 25 Sun, Yang (CR22) 2004 Ekrem, Willassen, Stur (CR5) 2007; 43 Purschke, Ding, Mfiller (CR19) 2005; 115 Fauchald (CR6) 1977; 28 Tamura, Kumar (CR23) 2002; 19 CR7 Liao (CR14) 2006 Moritz, Cicero (CR17) 2004; 2 Dasmahapatra, Mallet (CR3) 2006; 2006 Palumbi, Hillis, Moritz, Mable (CR18) 1996 Will, Rubinoff (CR26) 2004; 20 Yang, Sun (CR29) 1988 Hebert, Ratnasingham, deWaard (CR12) 2003; 270 Hebert, Penton, Burns, Janzen, Hallwachs (CR10) 2004; 101 Mikkelsen, Schander, Willassen (CR16) 2007; 36 Kim, Chae (CR13) 2001; 92 B. L. Wu (9131_CR28) 1981 P. D. N. Hebert (9131_CR10) 2004; 101 9131_CR7 M. Hajibabaei (9131_CR9) 2007; 23 S. R. Palumbi (9131_CR18) 1996 S. Ratnasingham (9131_CR20) 2007; 7 B. L. Wu (9131_CR27) 1997 K. W. Will (9131_CR26) 2004; 20 C. Antoniadou (9131_CR1) 2006; 60 C. Moritz (9131_CR17) 2004; 2 R. DeSalle (9131_CR4) 2005; 360 D. J. Yang (9131_CR29) 1988 K. Tamura (9131_CR23) 2002; 19 K. Tamura (9131_CR24) 2007; 24 K. Fauchald (9131_CR6) 1977; 28 P. D. N. Hebert (9131_CR11) 2003; 270 J. Kim (9131_CR13) 2001; 92 K. K. Dasmahapatra (9131_CR3) 2006; 2006 R. P. Sun (9131_CR22) 2004 N. T. Mikkelsen (9131_CR16) 2007; 36 K. R. Clarke (9131_CR2) 2001 G. Purschke (9131_CR19) 2005; 115 J. Zhou (9131_CR30) 2008 T. Ekrem (9131_CR5) 2007; 43 X. Z. Liao (9131_CR15) 2006; 25 C. Schander (9131_CR21) 2005; 1 P. D. N. Hebert (9131_CR12) 2003; 270 O. Folmer (9131_CR8) 1994; 3 J. D. Thompson (9131_CR25) 1994; 22 X. Z. Liao (9131_CR14) 2006 |
| References_xml | – volume: 115 start-page: 229 year: 2005 end-page: 241 ident: CR19 article-title: Ultrastructural differences as a taxonomic marker: the segmental ocelli of and sp. n. (Polychaeta, Opheliidae) publication-title: Zoomorphology doi: 10.1007/BF00393803 – year: 1997 ident: CR27 publication-title: Fauna Sinica: Phylum Annelida, Class Polychaeta, Order Phyllodocimorpha – volume: 92 start-page: 105 year: 2001 end-page: 111 ident: CR13 article-title: Optimized protocols for the detection of porcine circovirus DNA from formalin-fixed paraffin-embedded tissues using nested polymerase chain reaction and comparison of nested PCR with in situ hybridization publication-title: Journal of Virological Methods doi: 10.1016/S0166-0934(00)00255-X – start-page: 205 year: 1996 end-page: 247 ident: CR18 article-title: Nucleic acids II: the polymerase chain reaction publication-title: Molecular Systematics – volume: 7 start-page: 355 year: 2007 end-page: 364 ident: CR20 article-title: BOLD: The Barcode of Life Data System (www.barcodinglife.org) publication-title: Molecular Ecology Notes doi: 10.1111/j.1471-8286.2007.01678.x – volume: 23 start-page: 167 year: 2007 end-page: 172 ident: CR9 article-title: DNA barcoding: how it complements taxonomy, molecular phylogenetics and population genetics publication-title: Trends in Genetics doi: 10.1016/j.tig.2007.02.001 – volume: 270 start-page: 313 year: 2003 end-page: 321 ident: CR11 article-title: Biological identifications through DNA barcodes publication-title: Proc. R. Soc. Lond. B. Biol. Sci. doi: 10.1098/rspb.2002.2218 – volume: 25 start-page: 490 issue: 4 year: 2006 end-page: 497 ident: CR15 article-title: Phylogenetic analysis of polychaetes based on sequences of 18S rDNA and CO1 segments publication-title: Journal of Oceanography in Taiwan Strait – volume: 2 start-page: e279 issue: 10 year: 2004 end-page: e354 ident: CR17 article-title: DNA barcoding: promise and pitfalls publication-title: PLoS Biology doi: 10.1371/journal.pbio.0020354 – year: 2006 ident: CR14 publication-title: Molecular Phylogenetic Analysis of Marine Sedentary Polychaeta – year: 2008 ident: CR30 publication-title: Study on the Taxonomy and Faunistic Characters of Families Paraonidae and Spionidae (Annelida: Polychaeta) from China Seas – volume: 2006 start-page: 1 year: 2006 end-page: 2 ident: CR3 article-title: DNA barcodes: recent successes and future prospects publication-title: Heredity – volume: 19 start-page: 1 727 year: 2002 end-page: 1 736 ident: CR23 article-title: Evolutionary distance estimation under heterogeneous substitution pattern among lineages publication-title: Molecular Biology and Evolution – volume: 43 start-page: 530 year: 2007 end-page: 542 ident: CR5 article-title: A comprehensive DNA sequence library is essential for identification with DNA barcodes publication-title: Molecular Phylogenetics and Evolution doi: 10.1016/j.ympev.2006.11.021 – year: 1988 ident: CR29 publication-title: Polychaeta Annelids from China – volume: 22 start-page: 4 673 year: 1994 end-page: 4 680 ident: CR25 article-title: CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice publication-title: Nucleic Acids Res. doi: 10.1093/nar/22.22.4673 – year: 1981 ident: CR28 publication-title: Study on the Nereidae from China – year: 2001 ident: CR2 publication-title: Change in Marine Communities: An Approach to Statistical Analysis and Interpretation – volume: 36 start-page: 455 year: 2007 end-page: 463 ident: CR16 article-title: Local scale DNA barcoding of bivalves (Mollusca): a case study publication-title: Zoologica Scripta doi: 10.1111/j.1463-6409.2006.00289.x – year: 2004 ident: CR22 publication-title: Fauna Sinica: Invertebrata Vol. 33, Annelida, Polyhaeta II. Nereidida (= Nereimorpha) – volume: 270 start-page: S96 issue: Suppl.1 year: 2003 end-page: S99 ident: CR12 article-title: Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species publication-title: Proc. R. Soc. Lond. B. Biol. Sci. doi: 10.1098/rsbl.2003.0025 – volume: 1 start-page: 79 year: 2005 end-page: 83 ident: CR21 article-title: What can biological barcoding do for marine biology? publication-title: Marine Biology Research doi: 10.1080/17451000510018962 – volume: 24 start-page: 1 596 year: 2007 end-page: 1 599 ident: CR24 article-title: MEGA 4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0 publication-title: Molecular Biology and Evolution doi: 10.1093/molbev/msm092 – volume: 360 start-page: 1 905 year: 2005 end-page: 1 916 ident: CR4 article-title: The unholy trinity: taxonomy, species delimitation and DNA barcoding publication-title: Philosophical Transactions of the Royal Society B-Biological Sciences doi: 10.1098/rstb.2005.1722 – volume: 3 start-page: 294 year: 1994 end-page: 299 ident: CR8 article-title: DNA primers for amplification of mitochondrial cytochrome oxidase subunit I from diverse metazoan invertebrates publication-title: Mol. Mar. Biol. Biotechnol. – volume: 60 start-page: 39 year: 2006 end-page: 49 ident: CR1 article-title: Trophic relationships of polychaetes associated with different algal growth forms publication-title: Helgol. Mar. Res. doi: 10.1007/s10152-005-0015-2 – volume: 28 start-page: 1 year: 1977 end-page: 188 ident: CR6 article-title: The polychaete worms. Definitions and keys to the orders, families and genera publication-title: Nat. Hist. Mus. LA County Sci. Ser. – ident: CR7 – volume: 101 start-page: 14 812 year: 2004 end-page: 14 817 ident: CR10 article-title: Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly publication-title: Proceedings of the National Academy of Sciences of the United States of America doi: 10.1073/pnas.0406166101 – volume: 20 start-page: 47 year: 2004 end-page: 55 ident: CR26 article-title: Myth of the molecule: DNA barcodes for species cannot replace morphology for identification and classification publication-title: Cladistics doi: 10.1111/j.1096-0031.2003.00008.x – volume: 2 start-page: e279 issue: 10 year: 2004 ident: 9131_CR17 publication-title: PLoS Biology doi: 10.1371/journal.pbio.0020354 – volume: 115 start-page: 229 year: 2005 ident: 9131_CR19 publication-title: Zoomorphology doi: 10.1007/BF00393803 – volume: 28 start-page: 1 year: 1977 ident: 9131_CR6 publication-title: Nat. Hist. Mus. LA County Sci. Ser. – volume: 22 start-page: 4 673 year: 1994 ident: 9131_CR25 publication-title: Nucleic Acids Res. doi: 10.1093/nar/22.22.4673 – volume: 25 start-page: 490 issue: 4 year: 2006 ident: 9131_CR15 publication-title: Journal of Oceanography in Taiwan Strait – volume: 270 start-page: S96 issue: Suppl.1 year: 2003 ident: 9131_CR12 publication-title: Proc. R. Soc. Lond. B. Biol. Sci. – ident: 9131_CR7 – volume: 92 start-page: 105 year: 2001 ident: 9131_CR13 publication-title: Journal of Virological Methods doi: 10.1016/S0166-0934(00)00255-X – volume: 1 start-page: 79 year: 2005 ident: 9131_CR21 publication-title: Marine Biology Research doi: 10.1080/17451000510018962 – volume: 43 start-page: 530 year: 2007 ident: 9131_CR5 publication-title: Molecular Phylogenetics and Evolution doi: 10.1016/j.ympev.2006.11.021 – volume: 7 start-page: 355 year: 2007 ident: 9131_CR20 publication-title: Molecular Ecology Notes doi: 10.1111/j.1471-8286.2007.01678.x – volume-title: Study on the Taxonomy and Faunistic Characters of Families Paraonidae and Spionidae (Annelida: Polychaeta) from China Seas year: 2008 ident: 9131_CR30 – volume-title: Fauna Sinica: Phylum Annelida, Class Polychaeta, Order Phyllodocimorpha year: 1997 ident: 9131_CR27 – volume: 60 start-page: 39 year: 2006 ident: 9131_CR1 publication-title: Helgol. Mar. Res. doi: 10.1007/s10152-005-0015-2 – volume: 3 start-page: 294 year: 1994 ident: 9131_CR8 publication-title: Mol. Mar. Biol. Biotechnol. – volume-title: Molecular Phylogenetic Analysis of Marine Sedentary Polychaeta year: 2006 ident: 9131_CR14 – volume: 24 start-page: 1 596 year: 2007 ident: 9131_CR24 publication-title: Molecular Biology and Evolution doi: 10.1093/molbev/msm092 – volume: 360 start-page: 1 905 year: 2005 ident: 9131_CR4 publication-title: Philosophical Transactions of the Royal Society B-Biological Sciences doi: 10.1098/rstb.2005.1722 – volume-title: Study on the Nereidae from China year: 1981 ident: 9131_CR28 – volume-title: Polychaeta Annelids from China year: 1988 ident: 9131_CR29 – volume: 2006 start-page: 1 year: 2006 ident: 9131_CR3 publication-title: Heredity – volume: 36 start-page: 455 year: 2007 ident: 9131_CR16 publication-title: Zoologica Scripta doi: 10.1111/j.1463-6409.2006.00289.x – volume: 23 start-page: 167 year: 2007 ident: 9131_CR9 publication-title: Trends in Genetics doi: 10.1016/j.tig.2007.02.001 – volume: 101 start-page: 14 812 year: 2004 ident: 9131_CR10 publication-title: Proceedings of the National Academy of Sciences of the United States of America doi: 10.1073/pnas.0406166101 – volume: 270 start-page: 313 year: 2003 ident: 9131_CR11 publication-title: Proc. R. Soc. Lond. B. Biol. Sci. doi: 10.1098/rspb.2002.2218 – volume-title: Change in Marine Communities: An Approach to Statistical Analysis and Interpretation year: 2001 ident: 9131_CR2 – volume: 19 start-page: 1 727 year: 2002 ident: 9131_CR23 publication-title: Molecular Biology and Evolution doi: 10.1093/oxfordjournals.molbev.a003995 – volume-title: Fauna Sinica: Invertebrata Vol. 33, Annelida, Polyhaeta II. Nereidida (= Nereimorpha) year: 2004 ident: 9131_CR22 – start-page: 205 volume-title: Molecular Systematics year: 1996 ident: 9131_CR18 – volume: 20 start-page: 47 year: 2004 ident: 9131_CR26 publication-title: Cladistics doi: 10.1111/j.1096-0031.2003.00008.x |
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| Snippet | In this study,we integrated a DNA barcoding project with an ecological survey on intertidal polychaete communities and investigated the utility of CO1 gene... In this study, we integrated a DNA barcoding project with an ecological survey on intertidal polychaete communities and investigated the utility of CO1 gene... |
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| SubjectTerms | Animal populations Aquatic life Bar codes Deoxyribonucleic acid DNA Earth and Environmental Science Earth Sciences Genetic markers Marine Marine biology Oceanography rDNA序列 Worms 中国沿海 基因序列 多毛类 条形码 潮间带 物种鉴定 生态调查 |
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| Title | Integrating a DNA barcoding project with an ecological survey：a case study on temperate intertidal polychaete communities in Qingdao,China |
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