Green extraction of healthy and additive free mitochondria with a conventional centrifuge

• Published in: Lab on a chip Vol. 19; no. 22; pp. 3862 - 3869
• Main Authors: Lin, Ying-Ting, Chen, Sung-Tzu, Chang, Jui-Chih, Teoh, Ren-Jie, Liu, Chin-San, Wang, Gou-Jen
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 21.11.2019
• Subjects: Cell membranes; Centrifugal force; Disruption; Electroforming; Electron transport; Fibroblasts; Laboratories; Machining; Microfluidic devices; Mitochondria; Numerical controls; Photolithography; Polydimethylsiloxane; Proteins; Stainless steels; Transplantation

In this research, we propose a novel centrifugal device for the massive extraction of healthy mitochondria with a centrifuge used in general laboratories within 30 minutes. The device mainly consists of two key components. One component is a microfluidic device, which is fabricated by photolithograp...