Characterization of Methanosarcina mazeii TMA isolated from a paddy field soil

We isolated a methanogenic strain, designated as strain TMA (=DSM 9195), from an enrichment culture inoculated with a Japanese paddy field soil. Strain TMA was Gram positive and strictly anaerobic. Cell shape was pseudosarcina-like, and cells were nonmotile. The strain was able to use methylamines,...

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Published inCurrent microbiology Vol. 31; no. 1
Main Authors Asakawa, S. (Kyushu National Agricultural Experiment Station, Nishigoshi, Kumamoto, Japan.), Akagawa-Matsushita, M, Morii, H, Koga, Y, Hayano, K
Format Journal Article
LanguageEnglish
Published 01.07.1995
Subjects
BACTERIA
FERMENTACION METANICA
FERMENTATION METHANIQUE
FLORA DEL SUELO
FLORE DU SOL
IDENTIFICACION
IDENTIFICATION
JAPON
SOL DE RIZIERE
SUELO DE ARROZALES
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Summary:We isolated a methanogenic strain, designated as strain TMA (=DSM 9195), from an enrichment culture inoculated with a Japanese paddy field soil. Strain TMA was Gram positive and strictly anaerobic. Cell shape was pseudosarcina-like, and cells were nonmotile. The strain was able to use methylamines, methanol, H2-CO2, and acetate as substrates for methanogenesis, but did not utilize formate. The optimum temperature and optimum pH were 30-37 degrees C and 6.5-7.5 respectively. The G + C content of the DNA was 42.1 mol %. Strain TMA had DNA-DNA hybridization values of more than 80% with Methanosarcina mazeii S-6T (T = type strain). On the basis of phenotypic and genotypic characteristics, we identified strain TMA as M. mazeii. This is the first methylotrophic methanogen isolated from a paddy field soil and identified to the species level
Bibliography:P34
9556661
ISSN:0343-8651
1432-0991
DOI:10.1007/bf00294631