Lipase-Catalyzed Ring-Opening Polymerization of β-Butyrolactone: End-Group Analysis of Poly(3-hydroxybutanoate) Using Supercritical Fluid Chromatography

• Published in: Macromolecular bioscience Vol. 1; no. 5; pp. 171 - 177
• Main Authors: Osanai, Yasushi, Toshima, Kazunobu, Matsumura, Shuichi
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag GmbH 01.07.2001; WILEY‐VCH Verlag GmbH; Wiley-VCH
• Subjects: Applied sciences; Exact sciences and technology; Organic polymers; Physicochemistry of polymers; Polymerization; Preparation, kinetics, thermodynamics, mechanism and catalysts; Ring opening polymerization; Fractionation; End group; Bulk polymerization; Enzyme; Triacylglycerol lipase; Butyrate(hydroxy)polymer; Enzymatic catalysis; Esterases; Termination reaction; Transesterification; Experimental study; Lactone polymer; Carboxylic ester hydrolases; Supercritical fluid chromatography; Cyclic polymer; Linear polymer; Reaction mechanism; Hydrolases

The ring‐opening polymerization of (R,S)‐β‐butyrolactone (BL) in bulk was analyzed with respect to the polymer structure of the resulting poly[(R,S)‐3‐hydroxybutanoate)] [P(3HB)] by isolation of the pure form using preparative supercritical CO2 fluid chromatography. It was confirmed that the four‐me...