The effect of kinetic heat shock on bovine oocyte maturation and subsequent gene expression of targeted genes

• Published in: Zygote (Cambridge) Vol. 25; no. 3; pp. 383 - 389
• Main Authors: Pavani, Krishna C., Rocha, António, Baron, Erica, Lourenço, Joana, Faheem, Marwa, da Silva, Fernando Moreira
• Format: Journal Article
• Language: English
• Published: Cambridge, UK Cambridge University Press 01.06.2017
• Subjects: Animals; Apoptosis; Assaying; Cadherins - genetics; Cattle; Cell cycle; Connexin 43; Connexin 43 - genetics; Deoxyribonucleic acid; DNA; DNA (Cytosine-5-)-Methyltransferase 1 - genetics; DNA methylation; DNMT1 protein; E-cadherin; Embryos; Experiments; Female; Fertilization; Gene expression; Gene Expression Regulation; Gene regulation; Genes; Glyceraldehyde-3-phosphate dehydrogenase; Heat; Heat shock; Heat stress; Heat tolerance; Heat-Shock Response; HSP70 Heat-Shock Proteins - genetics; In Vitro Oocyte Maturation Techniques - methods; Maturation; Oocytes; Oocytes - physiology; Organelles; Proteins; Ribonucleic acid; RNA; Seasons; Short Communication; Stressing; Summer; Temperature effects; Winter; Kinetic heat shock; Gene quantification; Maternal heat stress; Apoptosis

The exposure of oocytes to heat stress during the maturation process results in harmful effects to their internal organelles, low fertilization capability and higher embryonic losses. In the present experiment the effect of heat shock (HS) during the maturation process was assessed. In Assay 1, oocy...