In vitro characterization of taurine transport using the human brain microvascular endothelial cell line as a human blood-brain barrier model
Taurine, a sulfur-containing β-amino acid, has various roles in the brain including cellular osmoregulation and neuroprotection. For adequate supply to the brain, taurine has to pass through the blood-brain barrier (BBB); however, the associated mechanism behind crossing the human BBB is not fully u...
Saved in:
| Published in | Drug metabolism and pharmacokinetics Vol. 61; p. 101040 |
|---|---|
| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
England
Elsevier Ltd
01.04.2025
|
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | Taurine, a sulfur-containing β-amino acid, has various roles in the brain including cellular osmoregulation and neuroprotection. For adequate supply to the brain, taurine has to pass through the blood-brain barrier (BBB); however, the associated mechanism behind crossing the human BBB is not fully understood. Therefore, we characterized taurine transport in vitro using the human brain microvascular endothelial (hCMEC/D3) cell line, a model of human BBB function. [3H]Taurine uptake by hCMEC/D3 cells exhibited time-, as well as extracellular Na+- and Cl−-dependence. The uptake was saturable with a Km of 19 μM and was inhibited by GABA at an IC50 of 328 μM, which were similar to Km values of taurine transporter (TauT)-mediated transport of taurine and GABA, respectively, suggesting that TauT is a major contributor to taurine uptake. For distribution to the brain, taurine must undergo cellular efflux after uptake. Taurine efflux from hCMEC/D3 cells increased for at least 60 min, and monocarboxylate transporter 7 (MCT7)-targeted siRNA significantly reduced MCT7 mRNA levels and [3H]taurine efflux by 93 % and 12 %, respectively, suggesting that MCT7 partly contributes to taurine efflux from hCMEC/D3 cells. Taken together, these results suggest that TauT and MCT7 function cooperatively in the human BBB.
[Display omitted] |
|---|---|
| AbstractList | Taurine, a sulfur-containing β-amino acid, has various roles in the brain including cellular osmoregulation and neuroprotection. For adequate supply to the brain, taurine has to pass through the blood-brain barrier (BBB); however, the associated mechanism behind crossing the human BBB is not fully understood. Therefore, we characterized taurine transport in vitro using the human brain microvascular endothelial (hCMEC/D3) cell line, a model of human BBB function. [
H]Taurine uptake by hCMEC/D3 cells exhibited time-, as well as extracellular Na
- and Cl
-dependence. The uptake was saturable with a K
of 19 μM and was inhibited by GABA at an IC
of 328 μM, which were similar to K
values of taurine transporter (TauT)-mediated transport of taurine and GABA, respectively, suggesting that TauT is a major contributor to taurine uptake. For distribution to the brain, taurine must undergo cellular efflux after uptake. Taurine efflux from hCMEC/D3 cells increased for at least 60 min, and monocarboxylate transporter 7 (MCT7)-targeted siRNA significantly reduced MCT7 mRNA levels and [
H]taurine efflux by 93 % and 12 %, respectively, suggesting that MCT7 partly contributes to taurine efflux from hCMEC/D3 cells. Taken together, these results suggest that TauT and MCT7 function cooperatively in the human BBB. Taurine, a sulfur-containing β-amino acid, has various roles in the brain including cellular osmoregulation and neuroprotection. For adequate supply to the brain, taurine has to pass through the blood-brain barrier (BBB); however, the associated mechanism behind crossing the human BBB is not fully understood. Therefore, we characterized taurine transport in vitro using the human brain microvascular endothelial (hCMEC/D3) cell line, a model of human BBB function. [3H]Taurine uptake by hCMEC/D3 cells exhibited time-, as well as extracellular Na+- and Cl−-dependence. The uptake was saturable with a Km of 19 μM and was inhibited by GABA at an IC50 of 328 μM, which were similar to Km values of taurine transporter (TauT)-mediated transport of taurine and GABA, respectively, suggesting that TauT is a major contributor to taurine uptake. For distribution to the brain, taurine must undergo cellular efflux after uptake. Taurine efflux from hCMEC/D3 cells increased for at least 60 min, and monocarboxylate transporter 7 (MCT7)-targeted siRNA significantly reduced MCT7 mRNA levels and [3H]taurine efflux by 93 % and 12 %, respectively, suggesting that MCT7 partly contributes to taurine efflux from hCMEC/D3 cells. Taken together, these results suggest that TauT and MCT7 function cooperatively in the human BBB. [Display omitted] Taurine, a sulfur-containing β-amino acid, has various roles in the brain including cellular osmoregulation and neuroprotection. For adequate supply to the brain, taurine has to pass through the blood-brain barrier (BBB); however, the associated mechanism behind crossing the human BBB is not fully understood. Therefore, we characterized taurine transport in vitro using the human brain microvascular endothelial (hCMEC/D3) cell line, a model of human BBB function. [3H]Taurine uptake by hCMEC/D3 cells exhibited time-, as well as extracellular Na+- and Cl--dependence. The uptake was saturable with a Km of 19 μM and was inhibited by GABA at an IC50 of 328 μM, which were similar to Km values of taurine transporter (TauT)-mediated transport of taurine and GABA, respectively, suggesting that TauT is a major contributor to taurine uptake. For distribution to the brain, taurine must undergo cellular efflux after uptake. Taurine efflux from hCMEC/D3 cells increased for at least 60 min, and monocarboxylate transporter 7 (MCT7)-targeted siRNA significantly reduced MCT7 mRNA levels and [3H]taurine efflux by 93 % and 12 %, respectively, suggesting that MCT7 partly contributes to taurine efflux from hCMEC/D3 cells. Taken together, these results suggest that TauT and MCT7 function cooperatively in the human BBB.Taurine, a sulfur-containing β-amino acid, has various roles in the brain including cellular osmoregulation and neuroprotection. For adequate supply to the brain, taurine has to pass through the blood-brain barrier (BBB); however, the associated mechanism behind crossing the human BBB is not fully understood. Therefore, we characterized taurine transport in vitro using the human brain microvascular endothelial (hCMEC/D3) cell line, a model of human BBB function. [3H]Taurine uptake by hCMEC/D3 cells exhibited time-, as well as extracellular Na+- and Cl--dependence. The uptake was saturable with a Km of 19 μM and was inhibited by GABA at an IC50 of 328 μM, which were similar to Km values of taurine transporter (TauT)-mediated transport of taurine and GABA, respectively, suggesting that TauT is a major contributor to taurine uptake. For distribution to the brain, taurine must undergo cellular efflux after uptake. Taurine efflux from hCMEC/D3 cells increased for at least 60 min, and monocarboxylate transporter 7 (MCT7)-targeted siRNA significantly reduced MCT7 mRNA levels and [3H]taurine efflux by 93 % and 12 %, respectively, suggesting that MCT7 partly contributes to taurine efflux from hCMEC/D3 cells. Taken together, these results suggest that TauT and MCT7 function cooperatively in the human BBB. |
| ArticleNumber | 101040 |
| Author | Inagaki, Mai Akanuma, Shin-ichi Kawauchi, Yusuke Tachikawa, Masanori Hosoya, Ken-ichi Tega, Yuma |
| Author_xml | – sequence: 1 givenname: Yuma orcidid: 0000-0003-4723-9089 surname: Tega fullname: Tega, Yuma organization: Department of Pharmaceutics, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan – sequence: 2 givenname: Yusuke surname: Kawauchi fullname: Kawauchi, Yusuke organization: Department of Pharmaceutics, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan – sequence: 3 givenname: Shin-ichi orcidid: 0000-0003-0996-8491 surname: Akanuma fullname: Akanuma, Shin-ichi email: akanumas@pha.u-toyama.ac.jp organization: Department of Pharmaceutics, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan – sequence: 4 givenname: Mai surname: Inagaki fullname: Inagaki, Mai organization: Graduate School of Biomedical Sciences, Tokushima University, Tokushima, Japan – sequence: 5 givenname: Masanori surname: Tachikawa fullname: Tachikawa, Masanori organization: Graduate School of Biomedical Sciences, Tokushima University, Tokushima, Japan – sequence: 6 givenname: Ken-ichi orcidid: 0000-0001-5633-3865 surname: Hosoya fullname: Hosoya, Ken-ichi organization: Department of Pharmaceutics, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/39923278$$D View this record in MEDLINE/PubMed |
| BookMark | eNp9kcuO1DAQRS00iHnAD7BAXrJJ40fSSUts0GiAkUZiA2urYlfTbhy7KTstwT_wzzjKDEtWVSqdW6q695pdxBSRsddSbKSQ23fHjZtOPzZKqHYZiFY8Y1dyGEQjdkpc1F63fdPqbX_JrnM-CqF116oX7FLvdkqrfrhif-4jP_tCidsDENiC5H9D8SnytOcFZvIReSGI-ZSo8Dn7-J2XA_LDPEHkI4GPfPKW0hmynQMQx-hSJYKHwC2GwMOyAzKHJ1FIyTWrdAQij8Sn5DC8ZM_3EDK-eqw37NvHu6-3n5uHL5_ubz88NFb3ujTY71qQelB6HEctOpS2tcop5QZrQQ9yqBOJziLayrSdlXtQojqj1RZH1Dfs7br3ROnnjLmYyeflVIiY5my03HZiEJ2QFX3ziM7jhM6cyE9Av8yThRVQK1AtyJlw_w-Rwiw5maNZcjJLTmbNqYreryKsX57r_yZbj9Gi84S2GJf8_-R_ATqEnf8 |
| Cites_doi | 10.1053/j.gastro.2004.08.017 10.1152/ajpgi.00388.2011 10.1007/BF00964232 10.1111/j.1748-1716.2006.01573.x 10.1007/s11064-014-1336-9 10.3390/nu13093220 10.1016/j.jbc.2022.101800 10.1016/0305-0491(85)90367-0 10.1097/00004647-200110000-00012 10.1111/j.1471-4159.2011.07208.x 10.1016/j.expneurol.2011.11.019 10.1042/bj3000893 10.3390/ijms24109003 10.1016/j.jconrel.2023.04.020 10.1074/jbc.M702111200 10.1038/s41586-021-04369-3 10.1248/bpb1978.4.879 10.1007/s00726-016-2244-x 10.1007/978-3-030-93337-1_32 10.1046/j.1471-4159.2002.01223.x 10.1096/fj.01-0691fje 10.1248/bpb.b17-00664 10.1113/jphysiol.2008.164228 10.1096/fj.03-0739com 10.1021/mp3004308 10.1016/0024-3205(77)90420-9 10.1248/bpb.b13-00991 10.1111/j.1432-1033.2004.04420.x 10.1016/j.jphs.2023.11.003 10.1002/jps.22487 10.1101/gad.180968.111 10.1016/0006-2952(95)02046-2 10.1016/j.bbamem.2006.10.001 10.1016/S0026-895X(24)12087-1 |
| ContentType | Journal Article |
| Copyright | 2024 The Japanese Society for the Study of Xenobiotics Copyright © 2024 The Japanese Society for the Study of Xenobiotics. Published by Elsevier Ltd. All rights reserved. |
| Copyright_xml | – notice: 2024 The Japanese Society for the Study of Xenobiotics – notice: Copyright © 2024 The Japanese Society for the Study of Xenobiotics. Published by Elsevier Ltd. All rights reserved. |
| DBID | 6I. AAFTH AAYXX CITATION CGR CUY CVF ECM EIF NPM 7X8 |
| DOI | 10.1016/j.dmpk.2024.101040 |
| DatabaseName | ScienceDirect Open Access Titles Elsevier:ScienceDirect:Open Access CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic |
| DatabaseTitle | CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE MEDLINE - Academic |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Pharmacy, Therapeutics, & Pharmacology |
| EISSN | 1880-0920 |
| ExternalDocumentID | 39923278 10_1016_j_dmpk_2024_101040 S1347436724000466 |
| Genre | Journal Article |
| GroupedDBID | --- --M 0R~ 29G 2WC 4.4 457 53G 5GY 6I. 7-5 8P~ AACTN AAEDT AAEDW AAFTH AAIKJ AAKOC AALRI AAOAW AAQFI AATTM AAXKI AAXUO ABJNI ABMAC ABXDB ACDAQ ACGFO ACGFS ACRLP ADBBV ADEZE AEBSH AEIPS AEKER AENEX AFJKZ AFTJW AFXIZ AGHFR AGUBO AIEXJ AIKHN AITUG AKRWK ALMA_UNASSIGNED_HOLDINGS AMRAJ ANKPU AXJTR BAWUL BKOJK BKOMP BLXMC CS3 DIK DU5 E3Z EBS EFJIC EJD F5P FDB FEDTE FIRID FYGXN GBLVA GX1 HH5 HVGLF HZ~ JMI JSF JSH KOM KQ8 M41 MOJWN O9- OAUVE RJT RNS ROL RZJ SPCBC SSP SSZ T5K TKC TR2 ~G- AAYWO AAYXX ACVFH ADCNI AEUPX AFPUW AGCQF AGRNS AIGII AIIUN AKBMS AKYEP APXCP BNPGV CITATION OVT SSH CGR CUY CVF ECM EFKBS EIF NPM 7X8 |
| ID | FETCH-LOGICAL-c373t-e794a13823bbb305e1c4c2d22d8cca3818e1c1edceec23b45c1fa20880326ebe3 |
| IEDL.DBID | AIKHN |
| ISSN | 1347-4367 1880-0920 |
| IngestDate | Mon Jul 21 10:44:03 EDT 2025 Mon Jul 21 05:57:32 EDT 2025 Sun Jul 06 05:01:24 EDT 2025 Sat Mar 29 16:12:19 EDT 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Keywords | Taurine hCMEC/D3 cell line Monocarboxylate transporter 7 Blood-brain barrier Taurine transporter |
| Language | English |
| License | This is an open access article under the CC BY-NC-ND license. Copyright © 2024 The Japanese Society for the Study of Xenobiotics. Published by Elsevier Ltd. All rights reserved. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c373t-e794a13823bbb305e1c4c2d22d8cca3818e1c1edceec23b45c1fa20880326ebe3 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ORCID | 0000-0001-5633-3865 0000-0003-4723-9089 0000-0003-0996-8491 |
| OpenAccessLink | https://www.sciencedirect.com/science/article/pii/S1347436724000466 |
| PMID | 39923278 |
| PQID | 3165080501 |
| PQPubID | 23479 |
| ParticipantIDs | proquest_miscellaneous_3165080501 pubmed_primary_39923278 crossref_primary_10_1016_j_dmpk_2024_101040 elsevier_sciencedirect_doi_10_1016_j_dmpk_2024_101040 |
| PublicationCentury | 2000 |
| PublicationDate | April 2025 2025-04-00 2025-Apr 20250401 |
| PublicationDateYYYYMMDD | 2025-04-01 |
| PublicationDate_xml | – month: 04 year: 2025 text: April 2025 |
| PublicationDecade | 2020 |
| PublicationPlace | England |
| PublicationPlace_xml | – name: England |
| PublicationTitle | Drug metabolism and pharmacokinetics |
| PublicationTitleAlternate | Drug Metab Pharmacokinet |
| PublicationYear | 2025 |
| Publisher | Elsevier Ltd |
| Publisher_xml | – name: Elsevier Ltd |
| References | Ohtsuki, Ikeda, Uchida, Sakamoto, Miller, Glacial (bib34) 2013; 10 Kang, Ohtsuki, Takanaga, Tomi, Hosoya, Terasaki (bib9) 2002; 83 Ito, Uchida, Ohtsuki, Aizawa, Kawakami, Katsukura (bib32) 2011; 100 Tamai, Senmaru, Terasaki, Tsuji (bib8) 1995; 50 Wang, Fan, Cai, Wu, Mo, Huang (bib2) 2016; 48 Higuchi, Sugiyama, Tomabechi, Kishimoto, Inoue (bib12) 2022; 298 bib15 Christiansen, Meinild, Jensen, Brauner-Osborne (bib31) 2007; 282 Voss, Pedersen, Christensen, Lambert (bib27) 2004; 271 Ikeda, Tachikawa, Akanuma, Fujinawa, Hosoya (bib23) 2012; 303 Heller-Stilb, van Roeyen, Rascher, Hartwig, Huth, Seeliger (bib6) 2002; 16 Tachikawa, Yokoyama, Akanuma, Hosoya (bib29) 2018; 41 Han, Patters, Jones, Zelikovic, Chesney (bib5) 2006; 187 Ramamoorthy, Leibach, Mahesh, Han, Yang-Feng, Blakely (bib22) 1994; 300 Yang, Vest, Kern, Lee, Agam, Maat (bib14) 2022; 603 Takanaga, Tokuda, Ohtsuki, Hosoya, Terasaki (bib35) 2002; 61 Tega, Kubo, Miura, Ri, Tomise, Akanuma (bib16) 2023; 24 Yamaoka, Tanigawara, Nakagawa, Uno (bib17) 1981; 4 Shukurova, Sadek, Mekawy, Bendaoud, Yachou, Mamchyn (bib3) 2022; 1370 Qi, Lin, Hu, Wei (bib21) 2023; 358 Al-Khawaja, Petersen, Damgaard, Jensen, Vogensen, Lie (bib30) 2014; 39 Thwaites, Anderson (bib24) 2007; 1768 Sturman, Rassin, Gaull (bib20) 1977; 21 Anderson, Howard, Walters, Ganapathy, Thwaites (bib26) 2009; 587 Higuchi, Sivaprakasam, Sennoune, Ogura, Bhutia, Rueda (bib13) 2021; 13 Yahara, Tachikawa, Akanuma, Kubo, Hosoya (bib28) 2014; 37 Pasantes-Morales, Chatagner, Mandel (bib4) 1980; 5 Takanaga, Ohtsuki, Hosoya, Terasaki (bib10) 2001; 21 Anderson, Grenade, Boll, Foltz, Wake, Kennedy (bib25) 2004; 127 Uchida, Ohtsuki, Katsukura, Ikeda, Suzuki, Kamiie (bib33) 2011; 117 Hugo, Cruz-Garcia, Karanth, Anderson, Stainier, Schlegel (bib11) 2012; 26 Louzada, Paula Lima, Mendonca-Silva, Noel, De Mello, Ferreira (bib1) 2004; 18 Miyazaki (bib19) 2024; 154 de la Rosa, Stipanuk (bib18) 1985; 81 Rasgado-Flores, Mokashi, Hawkins (bib7) 2012; 233 Takanaga (10.1016/j.dmpk.2024.101040_bib10) 2001; 21 Ito (10.1016/j.dmpk.2024.101040_bib32) 2011; 100 Han (10.1016/j.dmpk.2024.101040_bib5) 2006; 187 Qi (10.1016/j.dmpk.2024.101040_bib21) 2023; 358 Al-Khawaja (10.1016/j.dmpk.2024.101040_bib30) 2014; 39 Pasantes-Morales (10.1016/j.dmpk.2024.101040_bib4) 1980; 5 Tamai (10.1016/j.dmpk.2024.101040_bib8) 1995; 50 Kang (10.1016/j.dmpk.2024.101040_bib9) 2002; 83 Uchida (10.1016/j.dmpk.2024.101040_bib33) 2011; 117 Shukurova (10.1016/j.dmpk.2024.101040_bib3) 2022; 1370 Tachikawa (10.1016/j.dmpk.2024.101040_bib29) 2018; 41 Anderson (10.1016/j.dmpk.2024.101040_bib26) 2009; 587 Christiansen (10.1016/j.dmpk.2024.101040_bib31) 2007; 282 Ikeda (10.1016/j.dmpk.2024.101040_bib23) 2012; 303 Tega (10.1016/j.dmpk.2024.101040_bib16) 2023; 24 Ramamoorthy (10.1016/j.dmpk.2024.101040_bib22) 1994; 300 Wang (10.1016/j.dmpk.2024.101040_bib2) 2016; 48 Rasgado-Flores (10.1016/j.dmpk.2024.101040_bib7) 2012; 233 Higuchi (10.1016/j.dmpk.2024.101040_bib13) 2021; 13 Higuchi (10.1016/j.dmpk.2024.101040_bib12) 2022; 298 de la Rosa (10.1016/j.dmpk.2024.101040_bib18) 1985; 81 Ohtsuki (10.1016/j.dmpk.2024.101040_bib34) 2013; 10 Anderson (10.1016/j.dmpk.2024.101040_bib25) 2004; 127 Miyazaki (10.1016/j.dmpk.2024.101040_bib19) 2024; 154 Thwaites (10.1016/j.dmpk.2024.101040_bib24) 2007; 1768 Heller-Stilb (10.1016/j.dmpk.2024.101040_bib6) 2002; 16 Yang (10.1016/j.dmpk.2024.101040_bib14) 2022; 603 Yamaoka (10.1016/j.dmpk.2024.101040_bib17) 1981; 4 Hugo (10.1016/j.dmpk.2024.101040_bib11) 2012; 26 Yahara (10.1016/j.dmpk.2024.101040_bib28) 2014; 37 Louzada (10.1016/j.dmpk.2024.101040_bib1) 2004; 18 Sturman (10.1016/j.dmpk.2024.101040_bib20) 1977; 21 Voss (10.1016/j.dmpk.2024.101040_bib27) 2004; 271 Takanaga (10.1016/j.dmpk.2024.101040_bib35) 2002; 61 |
| References_xml | – volume: 117 start-page: 333 year: 2011 end-page: 345 ident: bib33 article-title: Quantitative targeted absolute proteomics of human blood-brain barrier transporters and receptors publication-title: J Neurochem – volume: 358 start-page: 78 year: 2023 end-page: 97 ident: bib21 article-title: A review on in vitro model of the blood-brain barrier (BBB) based on hCMEC/D3 cells publication-title: J Contr Release – volume: 18 start-page: 511 year: 2004 end-page: 518 ident: bib1 article-title: Taurine prevents the neurotoxicity of beta-amyloid and glutamate receptor agonists: activation of GABA receptors and possible implications for Alzheimer’s disease and other neurological disorders publication-title: Faseb J – volume: 13 start-page: 3220 year: 2021 ident: bib13 article-title: A proton-coupled transport system for beta-Hydroxy-beta-Methylbutyrate (HMB) in blood-brain barrier endothelial cell line hCMEC/D3 publication-title: Nutrients – volume: 100 start-page: 3939 year: 2011 end-page: 3950 ident: bib32 article-title: Quantitative membrane protein expression at the blood-brain barrier of adult and younger cynomolgus monkeys publication-title: J Pharm Sci – volume: 4 start-page: 879 year: 1981 end-page: 885 ident: bib17 article-title: A pharmacokinetic analysis program (multi) for microcomputer publication-title: J Pharmacobiodyn – volume: 26 start-page: 282 year: 2012 end-page: 293 ident: bib11 article-title: A monocarboxylate transporter required for hepatocyte secretion of ketone bodies during fasting publication-title: Genes Dev – volume: 187 start-page: 61 year: 2006 end-page: 73 ident: bib5 article-title: The taurine transporter: mechanisms of regulation publication-title: Acta Physiol – volume: 21 start-page: 1 year: 1977 end-page: 22 ident: bib20 article-title: Taurine in development publication-title: Life Sci – volume: 10 start-page: 289 year: 2013 end-page: 296 ident: bib34 article-title: Quantitative targeted absolute proteomic analysis of transporters, receptors and junction proteins for validation of human cerebral microvascular endothelial cell line hCMEC/D3 as a human blood-brain barrier model publication-title: Mol Pharm – volume: 1768 start-page: 179 year: 2007 end-page: 197 ident: bib24 article-title: Deciphering the mechanisms of intestinal imino (and amino) acid transport: the redemption of SLC36A1 publication-title: Biochim Biophys Acta – volume: 37 start-page: 817 year: 2014 end-page: 825 ident: bib28 article-title: Amino acid residues involved in the substrate specificity of TauT/SLC6A6 for taurine and gamma-aminobutyric acid publication-title: Biol Pharm Bull – volume: 61 start-page: 1289 year: 2002 end-page: 1296 ident: bib35 article-title: ATA2 is predominantly expressed as system A at the blood-brain barrier and acts as brain-to-blood efflux transport for L-proline publication-title: Mol Pharmacol – volume: 271 start-page: 4646 year: 2004 end-page: 4658 ident: bib27 article-title: Regulation of the expression and subcellular localization of the taurine transporter TauT in mouse NIH3T3 fibroblasts publication-title: Eur J Biochem – volume: 5 start-page: 441 year: 1980 end-page: 451 ident: bib4 article-title: Synthesis of taurine in rat liver and brain in vivo publication-title: Neurochem Res – volume: 48 start-page: 2169 year: 2016 end-page: 2177 ident: bib2 article-title: Protective effects of taurine in traumatic brain injury via mitochondria and cerebral blood flow publication-title: Amino Acids – volume: 50 start-page: 1783 year: 1995 end-page: 1793 ident: bib8 article-title: Na publication-title: Biochem Pharmacol – volume: 282 start-page: 19331 year: 2007 end-page: 19341 ident: bib31 article-title: Cloning and characterization of a functional human gamma-aminobutyric acid (GABA) transporter, human GAT-2 publication-title: J Biol Chem – volume: 41 start-page: 338 year: 2018 end-page: 341 ident: bib29 article-title: Assembly of taurine transporter (Slc6a6) with Na publication-title: Biol Pharm Bull – volume: 298 year: 2022 ident: bib12 article-title: Mammalian monocarboxylate transporter 7 (MCT7/Slc16a6) is a novel facilitative taurine transporter publication-title: J Biol Chem – volume: 39 start-page: 1988 year: 2014 end-page: 1996 ident: bib30 article-title: Pharmacological identification of a guanidine-containing beta-alanine analogue with low micromolar potency and selectivity for the betaine/GABA transporter 1 (BGT1) publication-title: Neurochem Res – volume: 21 start-page: 1232 year: 2001 end-page: 1239 ident: bib10 article-title: GAT2/BGT-1 as a system responsible for the transport of gamma-aminobutyric acid at the mouse blood-brain barrier publication-title: J Cereb Blood Flow Metab – ident: bib15 article-title: Human_BBB – volume: 300 start-page: 893 year: 1994 end-page: 900 ident: bib22 article-title: Functional characterization and chromosomal localization of a cloned taurine transporter from human placenta publication-title: Biochem J – volume: 83 start-page: 1188 year: 2002 end-page: 1195 ident: bib9 article-title: Regulation of taurine transport at the blood-brain barrier by tumor necrosis factor-alpha, taurine and hypertonicity publication-title: J Neurochem – volume: 1370 start-page: 333 year: 2022 end-page: 340 ident: bib3 article-title: Electrophysiological evidence for anti-epileptic property of taurine publication-title: Adv Exp Med Biol – volume: 127 start-page: 1410 year: 2004 end-page: 1422 ident: bib25 article-title: H publication-title: Gastroenterology – volume: 154 start-page: 9 year: 2024 end-page: 17 ident: bib19 article-title: Identification of a novel enzyme and the regulation of key enzymes in mammalian taurine synthesis publication-title: J Pharmacol Sci – volume: 233 start-page: 457 year: 2012 end-page: 462 ident: bib7 article-title: Na publication-title: Exp Neurol – volume: 603 start-page: 885 year: 2022 end-page: 892 ident: bib14 article-title: A human brain vascular atlas reveals diverse mediators of Alzheimer's risk publication-title: Nature – volume: 81 start-page: 565 year: 1985 end-page: 571 ident: bib18 article-title: Evidence for a rate-limiting role of cysteinesulfinate decarboxylase activity in taurine biosynthesis in vivo publication-title: Comp Biochem Physiol B – volume: 16 start-page: 231 year: 2002 end-page: 233 ident: bib6 article-title: Disruption of the taurine transporter gene (taut) leads to retinal degeneration in mice publication-title: Faseb J – volume: 24 start-page: 9003 year: 2023 ident: bib16 article-title: Carrier-mediated process of putrescine elimination at the rat blood-retinal barrier publication-title: Int J Mol Sci – volume: 303 start-page: G291 year: 2012 end-page: G297 ident: bib23 article-title: Involvement of gamma-aminobutyric acid transporter 2 in the hepatic uptake of taurine in rats publication-title: Am J Physiol Gastrointest Liver Physiol – volume: 587 start-page: 731 year: 2009 end-page: 744 ident: bib26 article-title: Taurine uptake across the human intestinal brush-border membrane is via two transporters: H publication-title: J Physiol – volume: 127 start-page: 1410 issue: 5 year: 2004 ident: 10.1016/j.dmpk.2024.101040_bib25 article-title: H+/amino acid transporter 1 (PAT1) is the imino acid carrier: an intestinal nutrient/drug transporter in human and rat publication-title: Gastroenterology doi: 10.1053/j.gastro.2004.08.017 – volume: 303 start-page: G291 issue: 3 year: 2012 ident: 10.1016/j.dmpk.2024.101040_bib23 article-title: Involvement of gamma-aminobutyric acid transporter 2 in the hepatic uptake of taurine in rats publication-title: Am J Physiol Gastrointest Liver Physiol doi: 10.1152/ajpgi.00388.2011 – volume: 5 start-page: 441 issue: 4 year: 1980 ident: 10.1016/j.dmpk.2024.101040_bib4 article-title: Synthesis of taurine in rat liver and brain in vivo publication-title: Neurochem Res doi: 10.1007/BF00964232 – volume: 187 start-page: 61 issue: 1–2 year: 2006 ident: 10.1016/j.dmpk.2024.101040_bib5 article-title: The taurine transporter: mechanisms of regulation publication-title: Acta Physiol doi: 10.1111/j.1748-1716.2006.01573.x – volume: 39 start-page: 1988 issue: 10 year: 2014 ident: 10.1016/j.dmpk.2024.101040_bib30 article-title: Pharmacological identification of a guanidine-containing beta-alanine analogue with low micromolar potency and selectivity for the betaine/GABA transporter 1 (BGT1) publication-title: Neurochem Res doi: 10.1007/s11064-014-1336-9 – volume: 13 start-page: 3220 issue: 9 year: 2021 ident: 10.1016/j.dmpk.2024.101040_bib13 article-title: A proton-coupled transport system for beta-Hydroxy-beta-Methylbutyrate (HMB) in blood-brain barrier endothelial cell line hCMEC/D3 publication-title: Nutrients doi: 10.3390/nu13093220 – volume: 298 issue: 4 year: 2022 ident: 10.1016/j.dmpk.2024.101040_bib12 article-title: Mammalian monocarboxylate transporter 7 (MCT7/Slc16a6) is a novel facilitative taurine transporter publication-title: J Biol Chem doi: 10.1016/j.jbc.2022.101800 – volume: 81 start-page: 565 issue: 3 year: 1985 ident: 10.1016/j.dmpk.2024.101040_bib18 article-title: Evidence for a rate-limiting role of cysteinesulfinate decarboxylase activity in taurine biosynthesis in vivo publication-title: Comp Biochem Physiol B doi: 10.1016/0305-0491(85)90367-0 – volume: 21 start-page: 1232 issue: 10 year: 2001 ident: 10.1016/j.dmpk.2024.101040_bib10 article-title: GAT2/BGT-1 as a system responsible for the transport of gamma-aminobutyric acid at the mouse blood-brain barrier publication-title: J Cereb Blood Flow Metab doi: 10.1097/00004647-200110000-00012 – volume: 117 start-page: 333 issue: 2 year: 2011 ident: 10.1016/j.dmpk.2024.101040_bib33 article-title: Quantitative targeted absolute proteomics of human blood-brain barrier transporters and receptors publication-title: J Neurochem doi: 10.1111/j.1471-4159.2011.07208.x – volume: 233 start-page: 457 issue: 1 year: 2012 ident: 10.1016/j.dmpk.2024.101040_bib7 article-title: Na+-dependent transport of taurine is found only on the abluminal membrane of the blood-brain barrier publication-title: Exp Neurol doi: 10.1016/j.expneurol.2011.11.019 – volume: 300 start-page: 893 issue: Pt 3 year: 1994 ident: 10.1016/j.dmpk.2024.101040_bib22 article-title: Functional characterization and chromosomal localization of a cloned taurine transporter from human placenta publication-title: Biochem J doi: 10.1042/bj3000893 – volume: 24 start-page: 9003 issue: 10 year: 2023 ident: 10.1016/j.dmpk.2024.101040_bib16 article-title: Carrier-mediated process of putrescine elimination at the rat blood-retinal barrier publication-title: Int J Mol Sci doi: 10.3390/ijms24109003 – volume: 358 start-page: 78 year: 2023 ident: 10.1016/j.dmpk.2024.101040_bib21 article-title: A review on in vitro model of the blood-brain barrier (BBB) based on hCMEC/D3 cells publication-title: J Contr Release doi: 10.1016/j.jconrel.2023.04.020 – volume: 282 start-page: 19331 issue: 27 year: 2007 ident: 10.1016/j.dmpk.2024.101040_bib31 article-title: Cloning and characterization of a functional human gamma-aminobutyric acid (GABA) transporter, human GAT-2 publication-title: J Biol Chem doi: 10.1074/jbc.M702111200 – volume: 603 start-page: 885 issue: 7903 year: 2022 ident: 10.1016/j.dmpk.2024.101040_bib14 article-title: A human brain vascular atlas reveals diverse mediators of Alzheimer's risk publication-title: Nature doi: 10.1038/s41586-021-04369-3 – volume: 4 start-page: 879 issue: 11 year: 1981 ident: 10.1016/j.dmpk.2024.101040_bib17 article-title: A pharmacokinetic analysis program (multi) for microcomputer publication-title: J Pharmacobiodyn doi: 10.1248/bpb1978.4.879 – volume: 48 start-page: 2169 issue: 9 year: 2016 ident: 10.1016/j.dmpk.2024.101040_bib2 article-title: Protective effects of taurine in traumatic brain injury via mitochondria and cerebral blood flow publication-title: Amino Acids doi: 10.1007/s00726-016-2244-x – volume: 1370 start-page: 333 year: 2022 ident: 10.1016/j.dmpk.2024.101040_bib3 article-title: Electrophysiological evidence for anti-epileptic property of taurine publication-title: Adv Exp Med Biol doi: 10.1007/978-3-030-93337-1_32 – volume: 83 start-page: 1188 issue: 5 year: 2002 ident: 10.1016/j.dmpk.2024.101040_bib9 article-title: Regulation of taurine transport at the blood-brain barrier by tumor necrosis factor-alpha, taurine and hypertonicity publication-title: J Neurochem doi: 10.1046/j.1471-4159.2002.01223.x – volume: 16 start-page: 231 issue: 2 year: 2002 ident: 10.1016/j.dmpk.2024.101040_bib6 article-title: Disruption of the taurine transporter gene (taut) leads to retinal degeneration in mice publication-title: Faseb J doi: 10.1096/fj.01-0691fje – volume: 41 start-page: 338 issue: 3 year: 2018 ident: 10.1016/j.dmpk.2024.101040_bib29 article-title: Assembly of taurine transporter (Slc6a6) with Na+-H+ exchanger regulatory factor 1 (Slc9a3r1) improves GABA transport activity by increasing the maximum transport velocity publication-title: Biol Pharm Bull doi: 10.1248/bpb.b17-00664 – volume: 587 start-page: 731 issue: Pt 4 year: 2009 ident: 10.1016/j.dmpk.2024.101040_bib26 article-title: Taurine uptake across the human intestinal brush-border membrane is via two transporters: H+-coupled PAT1 (SLC36A1) and Na+- and Cl--dependent TauT (SLC6A6) publication-title: J Physiol doi: 10.1113/jphysiol.2008.164228 – volume: 18 start-page: 511 issue: 3 year: 2004 ident: 10.1016/j.dmpk.2024.101040_bib1 article-title: Taurine prevents the neurotoxicity of beta-amyloid and glutamate receptor agonists: activation of GABA receptors and possible implications for Alzheimer’s disease and other neurological disorders publication-title: Faseb J doi: 10.1096/fj.03-0739com – volume: 10 start-page: 289 issue: 1 year: 2013 ident: 10.1016/j.dmpk.2024.101040_bib34 article-title: Quantitative targeted absolute proteomic analysis of transporters, receptors and junction proteins for validation of human cerebral microvascular endothelial cell line hCMEC/D3 as a human blood-brain barrier model publication-title: Mol Pharm doi: 10.1021/mp3004308 – volume: 21 start-page: 1 issue: 1 year: 1977 ident: 10.1016/j.dmpk.2024.101040_bib20 article-title: Taurine in development publication-title: Life Sci doi: 10.1016/0024-3205(77)90420-9 – volume: 37 start-page: 817 issue: 5 year: 2014 ident: 10.1016/j.dmpk.2024.101040_bib28 article-title: Amino acid residues involved in the substrate specificity of TauT/SLC6A6 for taurine and gamma-aminobutyric acid publication-title: Biol Pharm Bull doi: 10.1248/bpb.b13-00991 – volume: 271 start-page: 4646 issue: 23–24 year: 2004 ident: 10.1016/j.dmpk.2024.101040_bib27 article-title: Regulation of the expression and subcellular localization of the taurine transporter TauT in mouse NIH3T3 fibroblasts publication-title: Eur J Biochem doi: 10.1111/j.1432-1033.2004.04420.x – volume: 154 start-page: 9 issue: 1 year: 2024 ident: 10.1016/j.dmpk.2024.101040_bib19 article-title: Identification of a novel enzyme and the regulation of key enzymes in mammalian taurine synthesis publication-title: J Pharmacol Sci doi: 10.1016/j.jphs.2023.11.003 – volume: 100 start-page: 3939 issue: 9 year: 2011 ident: 10.1016/j.dmpk.2024.101040_bib32 article-title: Quantitative membrane protein expression at the blood-brain barrier of adult and younger cynomolgus monkeys publication-title: J Pharm Sci doi: 10.1002/jps.22487 – volume: 26 start-page: 282 issue: 3 year: 2012 ident: 10.1016/j.dmpk.2024.101040_bib11 article-title: A monocarboxylate transporter required for hepatocyte secretion of ketone bodies during fasting publication-title: Genes Dev doi: 10.1101/gad.180968.111 – volume: 50 start-page: 1783 issue: 11 year: 1995 ident: 10.1016/j.dmpk.2024.101040_bib8 article-title: Na+- and Cl--dependent transport of taurine at the blood-brain barrier publication-title: Biochem Pharmacol doi: 10.1016/0006-2952(95)02046-2 – volume: 1768 start-page: 179 issue: 2 year: 2007 ident: 10.1016/j.dmpk.2024.101040_bib24 article-title: Deciphering the mechanisms of intestinal imino (and amino) acid transport: the redemption of SLC36A1 publication-title: Biochim Biophys Acta doi: 10.1016/j.bbamem.2006.10.001 – volume: 61 start-page: 1289 issue: 6 year: 2002 ident: 10.1016/j.dmpk.2024.101040_bib35 article-title: ATA2 is predominantly expressed as system A at the blood-brain barrier and acts as brain-to-blood efflux transport for L-proline publication-title: Mol Pharmacol doi: 10.1016/S0026-895X(24)12087-1 |
| SSID | ssj0033542 |
| Score | 2.4039562 |
| Snippet | Taurine, a sulfur-containing β-amino acid, has various roles in the brain including cellular osmoregulation and neuroprotection. For adequate supply to the... |
| SourceID | proquest pubmed crossref elsevier |
| SourceType | Aggregation Database Index Database Publisher |
| StartPage | 101040 |
| SubjectTerms | Biological Transport - physiology Blood-brain barrier Blood-Brain Barrier - metabolism Brain - blood supply Brain - metabolism Cell Line Endothelial Cells - metabolism hCMEC/D3 cell line Humans Membrane Glycoproteins Membrane Transport Proteins - metabolism Microvessels - metabolism Monocarboxylate transporter 7 Monocarboxylic Acid Transporters - genetics Monocarboxylic Acid Transporters - metabolism Taurine Taurine - metabolism Taurine transporter |
| Title | In vitro characterization of taurine transport using the human brain microvascular endothelial cell line as a human blood-brain barrier model |
| URI | https://dx.doi.org/10.1016/j.dmpk.2024.101040 https://www.ncbi.nlm.nih.gov/pubmed/39923278 https://www.proquest.com/docview/3165080501 |
| Volume | 61 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1NS-RAEC10vHgR189Zd6UE8aJhku4kE48i6qggggpza_orMrpmhpko-CP8z9uVTiuC7mGvTTdpUtVV1cmr9wB2ZVlyXUgZMVXaKC0si2RqVaTYoSl1oZjSDcr3Kh_cpRfDbDgHx6EXhmCVbez3Mb2J1u1Ir32bvclo1LuhJsiU531CQbpbXj4PC8xl17gDC0fnl4OrEJA5zxoNHZof0YK2d8bDvMzT5NFdE1lKAzF9A_k6P31XfzZ56HQZltoCEo_8Hn_AnK1WYO_aM1C_HuDtR0PV7AD38PqDm_p1Fd7OK3wZ1dMx6nemZt-IieMSa-IYqizWgfEcCRZ_j65IxEbMDxUpSuATofgChhVtZaiN64_zZKT_AEiVK8oZyrCoAcf7pUpOSSMPGwWeNbg7Pbk9HkStIkOkeZ_XkXWnVxJrIVdKuUhhE51qZhgzhfMESv5uJCFgqdVuTprppJTMBbLYVYnOXfg6dKpxZTcBpc0LpqQhQaxU8UypuLB9yQ9zU5ikNF3YD3YQE0-8IQIi7UGQ1QRZTXirdSELphKf3Ee4zPDPdTvBrsKdK3pJsrLj55ngCdWucRYnXdjwBn_fB5H5ctYvfv7nU7dgkZGMcAMA-gWdevpsf7vaplbbre9uw_zZMPkLUnD80Q |
| linkProvider | Elsevier |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1Nb9QwEB2V9gAXxDfL5yBBLzTaxE6y6YFDBVS7bFlVYiv1ZmzHQUvb7Go3Be2P4NfwB5mJ41ZIwAGpVytOHD-PZ5y8eQPwUleVtIXWkTCVi9LCiUinzkRG7JaVLYwwtmX5TvLhUfrhODvegJ8hF4Zpld3e7_f0drfuWvrdbPYXs1n_EydBpjIfMAuSTnl5x6wcu_V3Oret3ozeEcivhNh_P307jLrSApGVA9lEjpahZvk9aYyhJe8Sm1pRClEW9ErsxaglYYaks3RNmtmk0oIsMqZwh95b0n2vwRarYZFZbe2NxsNJcABSZm3NHh5fxAPscnU8raw8W5zQsVSk3BDzN5c_-8O_xbut39u_BTe7gBX3_Jzchg1X34HtQ694vd7B6WUC12oHt_HwUgt7fRd-jGr8NmuWc7QXytA-8RPnFTasaVQ7bILCOjIN_wtSUIpt8UA0XMECz5g1GDiz6OqS08ZOyXKQ_zsgR8qoV6hDp5aM77saveSafNhW_LkHR1cC033YrOe1ewioXV4Io0suwJUamRkTF26g5W5eFmVSlT14HXBQCy_0oQID7qti1BSjpjxqPcgCVOq35arIE_2z34uAqyI75knStZufr5RMOFaOszjpwQMP-MU4WDxYikHx6D-f-hyuD6cfD9TBaDJ-DDcElzBuyUdPYLNZnrunFFc15lm3jhE-X7Xp_AIxjjnw |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=In+vitro+characterization+of+taurine+transport+using+the+human+brain+microvascular+endothelial+cell+line+as+a+human+blood-brain+barrier+model&rft.jtitle=Drug+metabolism+and+pharmacokinetics&rft.au=Tega%2C+Yuma&rft.au=Kawauchi%2C+Yusuke&rft.au=Akanuma%2C+Shin-ichi&rft.au=Inagaki%2C+Mai&rft.date=2025-04-01&rft.pub=Elsevier+Ltd&rft.issn=1347-4367&rft.volume=61&rft_id=info:doi/10.1016%2Fj.dmpk.2024.101040&rft.externalDocID=S1347436724000466 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1347-4367&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1347-4367&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1347-4367&client=summon |