Regulated degradation of an endoplasmic reticulum membrane protein in a tubular lysosome in Leishmania mexicana

• Published in: Molecular biology of the cell Vol. 12; no. 8; pp. 2364 - 2377
• Main Authors: Mullin, K A, Foth, B J, Ilgoutz, S C, Callaghan, J M, Zawadzki, J L, McFadden, G I, McConville, M J
• Format: Journal Article
• Language: English
• Published: United States The American Society for Cell Biology 01.08.2001
• Subjects: Animals; Cell Fractionation; Coloring Agents - metabolism; Endoplasmic Reticulum - enzymology; Glycosylphosphatidylinositols - metabolism; Humans; Hydrogen-Ion Concentration; Immunoblotting; Immunohistochemistry; Leishmania mexicana - enzymology; Leishmania mexicana - physiology; Leishmania mexicana - ultrastructure; Lysosomes - enzymology; Lysosomes - metabolism; Mannosyltransferases - genetics; Mannosyltransferases - metabolism; Microscopy, Confocal; Microtubules - metabolism; Microtubules - ultrastructure; Protein Transport - physiology; Recombinant Fusion Proteins - metabolism; Subcellular Fractions - metabolism

The cell surface of the human parasite Leishmania mexicana is coated with glycosylphosphatidylinositol (GPI)-anchored macromolecules and free GPI glycolipids. We have investigated the intracellular trafficking of green fluorescent protein- and hemagglutinin-tagged forms of dolichol-phosphate-mannose...