Response of Olfactory Sensory Neurons to Mercury Ions in Zebrafish: An Immunohistochemical Study

• Published in: Microscopy and microanalysis Vol. 28; no. 1; pp. 227 - 242
• Main Authors: Lazzari, Maurizio, Bettini, Simone, Milani, Liliana, Maurizii, Maria G., Franceschini, Valeria
• Format: Journal Article
• Language: English
• Published: New York, USA Cambridge University Press 01.02.2022; Oxford University Press
• Subjects: Animals; Biological Applications; Cell death; Cell density; Chloride; Epithelium; Exposure; Image analysis; Image processing; Immunohistochemistry; Ions - metabolism; Markers; Mercury; Mercury (metal); Mercury - metabolism; Neurons; Olfaction; Olfactory epithelium; Olfactory Mucosa; Olfactory receptor neurons; Olfactory Receptor Neurons - metabolism; Optical density; Pollutants; Sensory neurons; Subpopulations; Toxicity; Values; Zebrafish; Zebrafish - physiology; United States > US; olfactory epithelium; olfactory sensory neurons; immunohistochemistry; zebrafish; crypt cells; mercury ions
• ISSN: 1431-9276; 1435-8115
• DOI: 10.1017/S1431927621013763

Olfactory sensory neurons (OSNs) of fish belong to three main types: ciliated olfactory sensory neurons (cOSNs), microvillous olfactory sensory neurons (mOSNs), and crypt cells. Mercury is a toxic metal harmful for olfaction. We exposed the olfactory epithelium of zebrafish to three sublethal Hg2+ concentrations. Molecular markers specific for the different types of OSNs were immunohistochemically detected. Image analysis of treated sections enabled counting of marked cells and measurement of staining optical density indicative of the response of OSNs to Hg2+ exposure. The three types of OSNs reacted to mercury in a different way. Image analysis revealed that mOSNs are more susceptible to Hg2+ exposure than cOSNs and crypt cell density decreases. Moreover, while the ratio between sensory/nonsensory epithelium areas is unchanged, epithelium thickness drops, and dividing cells increase in the basal layer of the olfactory epithelium. Cell death but also reduction of apical processes and marker expression could account for changes in OSN immunostaining. Also, the differential results between dorsal and ventral halves of the olfactory rosette could derive from different water flows inside the olfactory chamber or different subpopulations in OSNs.