Rapid fabrication of electrochemical enzyme sensor chip using polydimethylsiloxane microfluidic channel

Applicability of polydimethylsiloxane (PDMS) for easy and rapid fabrication of enzyme sensor chips, based on electrochemical detection, is examined. The sensor chip consists of PDMS substrate with a microfluidic channel fabricated in it, and a glass substrate with enzyme-modified microelectrodes. Th...

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Bibliographic Details
Published inAnalytica chimica acta Vol. 468; no. 1; pp. 143 - 152
Main Authors Yamaguchi, Akira, Jin, Peng, Tsuchiyama, Hiroshi, Masuda, Takao, Sun, Kai, Matsuo, Shigeki, Misawa, Hiroaki
Format Journal Article
LanguageEnglish
Published Elsevier B.V 10.09.2002
Subjects
Enzyme sensor
Microchannel
Microelectrode
PDMS
Microelectrode
Enzyme sensor
Microchannel
PDMS
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Summary:Applicability of polydimethylsiloxane (PDMS) for easy and rapid fabrication of enzyme sensor chips, based on electrochemical detection, is examined. The sensor chip consists of PDMS substrate with a microfluidic channel fabricated in it, and a glass substrate with enzyme-modified microelectrodes. The two substrates are clamped together between plastic plates. The sensor chip has shown no leakage around the microelectrodes under continuous solution flow (34 μl/min). Amperometric response of the sensor chips developed in this work suggest that various types of enzyme sensors can be designed by using PDMS microfluidic channels.
ISSN:0003-2670
1873-4324
DOI:10.1016/S0003-2670(02)00634-7