Aldehyde dehydrogenase in fresh primordial germ cells as a marker of cell ‘stemness’

• Published in: Zygote (Cambridge) Vol. 27; no. 1; pp. 46 - 48
• Main Authors: Svoradová, Andrea, Vašíček, Jaromír, Ostró, Alexander, Chrenek, Peter
• Format: Journal Article
• Language: English
• Published: Cambridge, UK Cambridge University Press 01.02.2019
• Subjects: Aldehyde dehydrogenase; Aldehyde Dehydrogenase - metabolism; Animals; Antigens; Biomarkers - metabolism; Chickens; Dehydrogenase; Dehydrogenases; Flow Cytometry; Fluorescence; Germ cells; Germ Cells - cytology; Germ Cells - metabolism; Inhibitors; Laboratory animals; Pluripotency; Poultry; R&D; Research & development; Short Communication; Stem cells; Stem Cells - metabolism; United States > US; Germany; Slovakia; ALDH; Flow cytometry; Chicken; Primordial germ cells; ALDEFLUOR

Chicken primordial germ cells (PGCs) are the primary pluripotent stem cell types that will differentiate towards germ cells. High aldehyde dehydrogenase (ALDH) activity is considered as a functional marker for the detection of cell ‘stemness’. In our study the ALDEFLUOR™ kit was used for determinati...