Acetazolamide loaded-silver nanoparticles: A potential treatment for murine trichinellosis

• Published in: Journal of helminthology Vol. 97; p. e86
• Main Authors: Abdel Hamed, E F, Taha, A A, Abdel Ghany, S M, Saleh, A A, Fawzy, E M
• Format: Journal Article
• Language: English
• Published: England Cambridge University Press 16.11.2023
• Subjects: Acetazolamide; Acetazolamide - pharmacology; Acetazolamide - therapeutic use; Adults; Albinism; Animals; Antioxidants; Antioxidants - pharmacology; Bioavailability; Drug dosages; Encephalitis; Enzymes; Foodborne diseases; Histopathology; Infections; Inflammation; Intestine; Larva; Larvae; Male; Metal Nanoparticles - therapeutic use; Mice; Mortality; Nanoparticles; Oxidative stress; Parasites; Parasitic diseases; Protozoa; Rodent Diseases; Scanning electron microscopy; Scavenging; Serum; Silver; Silver - pharmacology; Therapy; Trichinella spiralis; Trichinellosis; Trichinellosis - parasitology; Viviparity; Worms; AgNPs; Trichinella spiralis; antioxidant capacity; acetazolamide
• DOI: 10.1017/S0022149X23000731
• ISSN: 0022-149X

Trichinellosis is a global food-borne disease caused by viviparous parasitic nematodes of the genus Due to the lack of effective, safe therapy and the documented adverse effects of traditional therapy, this study aimed to evaluate the therapeutic effect of acetazolamide-loaded silver nanoparticles (AgNPs) on murine trichinellosis. Fifty male Swiss albino mice were divided into five groups of ten mice each: Group I, normal control group; Group II, infected with and not treated; Group III, infected and given AgNPs; Group IV, infected and treated with acetazolamide; and Group V, infected and treated with acetazolamide-loaded AgNPs. Mice were infected orally with 250 larvae. The efficacy was assessed by counting adults and larvae, measuring serum total antioxidant capacity, and observing the histopathological and ultrastructural alterations. Acetazolamide-loaded AgNPs treatment exhibited the highest percentage of reduction (84.72% and 80.74%) for the intestinal adults and the muscular larvae of infected animals, respectively. Furthermore, during the intestinal and muscular phases, the serum of the same group had the best free-radical scavenging capacity (antioxidant capacity), which reduced tissue damage induced by oxidative stress. Histopathologically, the normal intestinal and muscular architecture was restored in the group treated with acetazolamide-loaded AgNPs, in addition to the reduced inflammatory infiltrate that alleviated inflammation compared to infected animals. Our results confirmed the marked destruction of the ultrastructural features of adults and larvae. Acetazolamide-loaded AgNPs are a promising therapy against infection.