UHRF1 depletion causes a G2/M arrest, activation of DNA damage response and apoptosis

• Published in: Biochemical journal Vol. 435; no. 1; p. 175
• Main Authors: Tien, Amy L, Senbanerjee, Sucharita, Kulkarni, Atul, Mudbhary, Raksha, Goudreau, Bernadette, Ganesan, Shridar, Sadler, Kirsten C, Ukomadu, Chinweike
• Format: Journal Article
• Language: English
• Published: England 01.04.2011
• Subjects: Apoptosis; Caspase 3 - metabolism; Caspase 8 - genetics; Caspase 8 - metabolism; CCAAT-Enhancer-Binding Proteins - genetics; CCAAT-Enhancer-Binding Proteins - metabolism; CDC2 Protein Kinase - metabolism; cdc25 Phosphatases - metabolism; Cell Line, Tumor; Checkpoint Kinase 2; DNA Damage - radiation effects; G2 Phase; Gene Silencing; Histones - metabolism; Humans; In Situ Nick-End Labeling; Phosphorylation; Protein Processing, Post-Translational; Protein Serine-Threonine Kinases - metabolism; Protein Transport; RNA, Messenger - metabolism; RNA, Small Interfering; Ubiquitin-Protein Ligases; Ultraviolet Rays - adverse effects

UHRF1 [ubiquitin-like protein, containing PHD (plant homeodomain) and RING finger domains 1] is required for cell cycle progression and epigenetic regulation. In the present study, we show that depleting cancer cells of UHRF1 causes activation of the DNA damage response pathway, cell cycle arrest in...