Purification and Partial Characterization of an Acidic Polysaccharide with Complement Fixing Ability from the Stems of Avicennia Marina

An acidic polysaccharide fraction that had high anti-complementary activity was isolated from the stems of Grey Mangrove in 0.15% yield. The final fractions was designated HAM-3-IIb-II. The polysaccharide fraction appeared to be homogenous by high performance size exclusion chromatography with an es...

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Published inBMB reports Vol. 39; no. 5; pp. 546 - 555
Main Authors Fang, Xubo (Southern Yangtze University, Wuxi, China), Jiang, Bo (Southern Yangtze University, Wuxi, China), E-mail: bjiang@sytu.edu.cn, Wang, Xiaolan (Southern Yangtze University, Wuxi, China)
Format Journal Article
LanguageEnglish
Published Korea (South) 30.09.2006
Subjects
Animals
Anti-complementary activity
Arabinogalactans
Avicennia - chemistry
Avicennia marina
Complement Activation
Complement Fixation Tests
Complement Inactivator Proteins - chemistry
Complement Inactivator Proteins - immunology
Complement Inactivator Proteins - isolation & purification
Complement System Proteins - metabolism
Erythrocytes
Humans
PECTINAS
PECTINE
PECTINS
Plant Extracts - chemistry
Plant Stems - chemistry
POLISACARIDOS
POLYHOLOSIDE
POLYSACCHARIDES
Polysaccharides - chemistry
Polysaccharides - immunology
Polysaccharides - isolation & purification
Sheep
Spectroscopy, Fourier Transform Infrared
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Abstract An acidic polysaccharide fraction that had high anti-complementary activity was isolated from the stems of Grey Mangrove in 0.15% yield. The final fractions was designated HAM-3-IIb-II. The polysaccharide fraction appeared to be homogenous by high performance size exclusion chromatography with an estimated molecular weight of 105 kDa. The isolated polysaccharide is more effective than polysaccharide K (PSK) in its anti-complementary activity at 58 ㎍/ml of PSK and 23 ㎍/ml of HAM-3-IIb-II that inhibit 50% of complement activity in the complement fixation assay.
AbstractList An acidic polysaccharide fraction that had high anti-complementary activity was isolated from the stems of Grey Mangrove in 0.15% yield. The final fractions was designated HAM-3-IIb-II. The polysaccharide fraction appeared to be homogenous by high performance size exclusion chromatography with an estimated molecular weight of 105 kDa. The isolated polysaccharide is more effective than polysaccharide K (PSK) in its anti-complementary activity at 58 ㎍/ml of PSK and 23 ㎍/ml of HAM-3-IIb-II that inhibit 50% of complement activity in the complement fixation assay.
An acidic polysaccharide fraction that had high anticomplementary activity was isolated from the stems of Grey Mangrove in 0.15% yield. The final fractions was designated HAM-3-IIb-II. The polysaccharide fraction appeared to be homogenous by high performance size exclusion chromatography with an estimated molecular weight of 105 kDa. The isolated polysaccharide is more effective than polysaccharide K (PSK) in its anticomplementary activity at 58 microg/ml of PSK and 23 microg/ml of HAM-3-IIb-II that inhibit 50% of complement activity in the complement fixation assay. Structural studies indicated that HAM-3-IIb-II was rich in galacturonic acid along with arabinose, galactose and rhamnose, characterizing a pectin-type polysaccharide, which was also confirmed by FT-IR spectrum. The presence of rich neutral sugar side chains of arabinogalactans may have contributed to the expression of high activity. Traditionally, this mangrove plant is used for medicinal purposes and it appears to have some scientific applications.
Author Fang, Xubo (Southern Yangtze University, Wuxi, China)
Wang, Xiaolan (Southern Yangtze University, Wuxi, China)
Jiang, Bo (Southern Yangtze University, Wuxi, China), E-mail: bjiang@sytu.edu.cn
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Cites_doi 10.1016/0003-2697(73)90377-1
10.1023/A:1021397624349
10.1016/S0308-8146(99)00191-0
10.1016/S0166-3542(99)00058-3
10.1016/S0144-8617(00)00151-X
10.1055/s-2006-960205
10.1248/bpb.25.1203
10.1016/0008-6215(89)84098-4
10.1016/0008-6215(83)88244-5
10.1002/eji.1830210103
10.1016/0008-6215(91)89049-L
10.1016/j.bmc.2004.08.003
10.1016/j.carres.2004.10.023
10.1016/S0144-8617(97)00238-5
10.1016/0019-2791(76)90181-6
10.1016/j.carres.2003.11.022
10.1016/S0144-8617(96)00036-7
10.1016/S0367-326X(99)00169-0
10.1016/j.phytochem.2005.03.016
10.1021/ac60111a017
10.1016/S0378-8741(02)00330-6
10.1016/0144-8617(94)90052-3
10.1016/S0144-8617(98)00066-6
10.1016/S0378-8741(99)00112-9
10.1016/j.carbpol.2004.07.018
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  doi: 10.1248/bpb.25.1203
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  doi: 10.1016/0008-6215(89)84098-4
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  doi: 10.1016/0008-6215(83)88244-5
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  doi: 10.1002/eji.1830210103
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  doi: 10.1016/j.carres.2004.10.023
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  doi: 10.1016/S0144-8617(97)00238-5
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  doi: 10.1016/0019-2791(76)90181-6
– ident: key-10.5483/BMBRep.2006.39.5.546-11
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  doi: 10.1021/ac60111a017
– ident: key-10.5483/BMBRep.2006.39.5.546-7
  doi: 10.1016/S0378-8741(02)00330-6
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  doi: 10.1016/0144-8617(94)90052-3
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  doi: 10.1016/S0378-8741(99)00112-9
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Snippet An acidic polysaccharide fraction that had high anti-complementary activity was isolated from the stems of Grey Mangrove in 0.15% yield. The final fractions...
An acidic polysaccharide fraction that had high anticomplementary activity was isolated from the stems of Grey Mangrove in 0.15% yield. The final fractions was...
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SubjectTerms Animals
Anti-complementary activity
Arabinogalactans
Avicennia - chemistry
Avicennia marina
Complement Activation
Complement Fixation Tests
Complement Inactivator Proteins - chemistry
Complement Inactivator Proteins - immunology
Complement Inactivator Proteins - isolation & purification
Complement System Proteins - metabolism
Erythrocytes
Humans
PECTINAS
PECTINE
PECTINS
Plant Extracts - chemistry
Plant Stems - chemistry
POLISACARIDOS
POLYHOLOSIDE
POLYSACCHARIDES
Polysaccharides - chemistry
Polysaccharides - immunology
Polysaccharides - isolation & purification
Sheep
Spectroscopy, Fourier Transform Infrared
Title Purification and Partial Characterization of an Acidic Polysaccharide with Complement Fixing Ability from the Stems of Avicennia Marina
URI https://www.ncbi.nlm.nih.gov/pubmed/17002875
Volume 39
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