Purification and Partial Characterization of an Acidic Polysaccharide with Complement Fixing Ability from the Stems of Avicennia Marina

• Published in: BMB reports Vol. 39; no. 5; pp. 546 - 555
• Main Authors: Fang, Xubo (Southern Yangtze University, Wuxi, China), Jiang, Bo (Southern Yangtze University, Wuxi, China), E-mail: bjiang@sytu.edu.cn, Wang, Xiaolan (Southern Yangtze University, Wuxi, China)
• Format: Journal Article
• Language: English
• Published: Korea (South) 30.09.2006
• Subjects: Animals; Anti-complementary activity; Arabinogalactans; Avicennia - chemistry; Avicennia marina; Complement Activation; Complement Fixation Tests; Complement Inactivator Proteins - chemistry; Complement Inactivator Proteins - immunology; Complement Inactivator Proteins - isolation & purification; Complement System Proteins - metabolism; Erythrocytes; Humans; PECTINAS; PECTINE; PECTINS; Plant Extracts - chemistry; Plant Stems - chemistry; POLISACARIDOS; POLYHOLOSIDE; POLYSACCHARIDES; Polysaccharides - chemistry; Polysaccharides - immunology; Polysaccharides - isolation & purification; Sheep; Spectroscopy, Fourier Transform Infrared
• ISSN: 1225-8687; 1976-6696; 0219-1024
• DOI: 10.5483/BMBRep.2006.39.5.546

An acidic polysaccharide fraction that had high anti-complementary activity was isolated from the stems of Grey Mangrove in 0.15% yield. The final fractions was designated HAM-3-IIb-II. The polysaccharide fraction appeared to be homogenous by high performance size exclusion chromatography with an estimated molecular weight of 105 kDa. The isolated polysaccharide is more effective than polysaccharide K (PSK) in its anti-complementary activity at 58 ㎍/ml of PSK and 23 ㎍/ml of HAM-3-IIb-II that inhibit 50% of complement activity in the complement fixation assay.