A Systematic Literature Review of Microscopy Methods Reported in Malaria Clinical Trials

Microscopy of stained blood films is essential for the diagnosis of malaria, differentiation of parasite species, and estimation of parasite density performed for assessments of antimalarial drug efficacy. The accuracy and comparability of these measures over time and space are vital to discern the...

Full description

Saved in:
Bibliographic Details
Published inThe American journal of tropical medicine and hygiene Vol. 104; no. 3; pp. 836 - 841
Main Authors Das, Debashish, Dahal, Prabin, Dhorda, Mehul, Citarella, Barbara Wanjiru, Kennon, Kalynn, Stepniewska, Kasia, Felger, Ingrid, Chappuis, François, Guerin, Philippe J.
Format Journal Article
LanguageEnglish
Published United States Institute of Tropical Medicine 01.03.2021
The American Society of Tropical Medicine and Hygiene
Subjects
Clinical trials
Clinical Trials as Topic
Drug efficacy
Humans
Malaria
Malaria - diagnosis
Medical diagnosis
Methods
Microscopy
Microscopy - methods
Parasites
Review
Online AccessGet full text

Cover

Abstract Microscopy of stained blood films is essential for the diagnosis of malaria, differentiation of parasite species, and estimation of parasite density performed for assessments of antimalarial drug efficacy. The accuracy and comparability of these measures over time and space are vital to discern the emergence or spread of antimalarial drug resistance. Although evidence-based guidelines for malaria microscopy methods exist, the age-old microscopy techniques for parasitological assessments are subject to considerable methodological variations. The purpose of this review was to explore critically how microscopy methods were reported in published malarial studies between 2013 and 2017 with the focus on outlining the methodological differences and improving reporting standards in practice.
AbstractList Microscopy of stained blood films is essential for the diagnosis of malaria, differentiation of parasite species, and estimation of parasite density performed for assessments of antimalarial drug efficacy. The accuracy and comparability of these measures over time and space are vital to discern the emergence or spread of antimalarial drug resistance. Although evidence-based guidelines for malaria microscopy methods exist, the age-old microscopy techniques for parasitological assessments are subject to considerable methodological variations. The purpose of this review was to explore critically how microscopy methods were reported in published malarial studies between 2013 and 2017 with the focus on outlining the methodological differences and improving reporting standards in practice.
Microscopy of stained blood films is essential for the diagnosis of malaria, differentiation of parasite species, and estimation of parasite density performed for assessments of antimalarial drug efficacy. The accuracy and comparability of these measures over time and space are vital to discern the emergence or spread of antimalarial drug resistance. Although evidence-based guidelines for malaria microscopy methods exist, the age-old microscopy techniques for parasitological assessments are subject to considerable methodological variations. The purpose of this review was to explore critically how microscopy methods were reported in published malarial studies between 2013 and 2017 with the focus on outlining the methodological differences and improving reporting standards in practice.Microscopy of stained blood films is essential for the diagnosis of malaria, differentiation of parasite species, and estimation of parasite density performed for assessments of antimalarial drug efficacy. The accuracy and comparability of these measures over time and space are vital to discern the emergence or spread of antimalarial drug resistance. Although evidence-based guidelines for malaria microscopy methods exist, the age-old microscopy techniques for parasitological assessments are subject to considerable methodological variations. The purpose of this review was to explore critically how microscopy methods were reported in published malarial studies between 2013 and 2017 with the focus on outlining the methodological differences and improving reporting standards in practice.
Author Dhorda, Mehul
Citarella, Barbara Wanjiru
Das, Debashish
Stepniewska, Kasia
Kennon, Kalynn
Guerin, Philippe J.
Dahal, Prabin
Felger, Ingrid
Chappuis, François
Author_xml – sequence: 1
  givenname: Debashish
  surname: Das
  fullname: Das, Debashish
  organization: 1Institute of Global Health, University of Geneva, Geneva, Switzerland;, 2Infectious Diseases Data Observatory (IDDO), Oxford, United Kingdom;, 3Nuffield Department of Medicine, Centre for Tropical Medicine and Global Health, University of Oxford, Oxford, United Kingdom
– sequence: 2
  givenname: Prabin
  surname: Dahal
  fullname: Dahal, Prabin
  organization: 2Infectious Diseases Data Observatory (IDDO), Oxford, United Kingdom;, 3Nuffield Department of Medicine, Centre for Tropical Medicine and Global Health, University of Oxford, Oxford, United Kingdom
– sequence: 3
  givenname: Mehul
  surname: Dhorda
  fullname: Dhorda, Mehul
  organization: 2Infectious Diseases Data Observatory (IDDO), Oxford, United Kingdom;, 3Nuffield Department of Medicine, Centre for Tropical Medicine and Global Health, University of Oxford, Oxford, United Kingdom
– sequence: 4
  givenname: Barbara Wanjiru
  surname: Citarella
  fullname: Citarella, Barbara Wanjiru
  organization: 2Infectious Diseases Data Observatory (IDDO), Oxford, United Kingdom;, 3Nuffield Department of Medicine, Centre for Tropical Medicine and Global Health, University of Oxford, Oxford, United Kingdom
– sequence: 5
  givenname: Kalynn
  surname: Kennon
  fullname: Kennon, Kalynn
  organization: 2Infectious Diseases Data Observatory (IDDO), Oxford, United Kingdom;, 3Nuffield Department of Medicine, Centre for Tropical Medicine and Global Health, University of Oxford, Oxford, United Kingdom
– sequence: 6
  givenname: Kasia
  surname: Stepniewska
  fullname: Stepniewska, Kasia
  organization: 2Infectious Diseases Data Observatory (IDDO), Oxford, United Kingdom;, 3Nuffield Department of Medicine, Centre for Tropical Medicine and Global Health, University of Oxford, Oxford, United Kingdom
– sequence: 7
  givenname: Ingrid
  surname: Felger
  fullname: Felger, Ingrid
  organization: 4Swiss Tropical and Public Health Institute, Basel, Switzerland;, 5University of Basel, Basel, Switzerland
– sequence: 8
  givenname: François
  surname: Chappuis
  fullname: Chappuis, François
  organization: 6Division of Tropical and Humanitarian Medicine, Geneva University Hospitals and University of Geneva, Geneva, Switzerland
– sequence: 9
  givenname: Philippe J.
  surname: Guerin
  fullname: Guerin, Philippe J.
  organization: 2Infectious Diseases Data Observatory (IDDO), Oxford, United Kingdom;, 3Nuffield Department of Medicine, Centre for Tropical Medicine and Global Health, University of Oxford, Oxford, United Kingdom
BackLink https://www.ncbi.nlm.nih.gov/pubmed/33350371$$D View this record in MEDLINE/PubMed
BookMark eNp9kttrFDEUxoNU7Lb66KsEfPFlaq6TmRehLPUCuwhawbeQyZy4WWYma5Kp7H9vxraiRXw6hPM7X75zOUMnU5gAoeeUXAhWt6_NPo-7C0Yqymj7CK2oUHVFayFP0IoQwqq25uoUnaW0J4Q2jNIn6JRzLglXdIW-XuLPx5RhNNlbvPEZoslzBPwJbjz8wMHhrbcxJBsOR7yFvAt9KslDiBl67Ce8NYOJ3uD14CdvzYCvy2tIT9FjVwI8u4vn6Mvbq-v1-2rz8d2H9eWmslzxXPHOSeCGddx2nSBcSuWkU8WoAyNc44zoG24tNL2VHQVgghJKbU2MbLhi_By9udU9zN0IvYUpRzPoQ_SjiUcdjNd_Zya_09_CjVatoA1vi8CrO4EYvs-Qsh59sjAMZoIwJ82EYpQ0rF7-evkA3Yc5TqU9zWRNBOWEif9TrJGEyJYX6sWfvn8bvl9NAfgtsEw_RXDa-ly2FJY2_KAp0csB6F8HoBnRywGUqupB1b3wv_mfhdCzGA
CitedBy_id crossref_primary_10_11648_j_ajbls_20241204_11
crossref_primary_10_1080_23144599_2022_2111514
crossref_primary_10_12688_wellcomeopenres_17468_1
crossref_primary_10_1155_2022_7324281
crossref_primary_10_1186_s12936_023_04583_6
crossref_primary_10_1371_journal_pgph_0003091
ContentType Journal Article
Copyright Copyright Institute of Tropical Medicine Mar 2021
The American Society of Tropical Medicine and Hygiene 2021
Copyright_xml – notice: Copyright Institute of Tropical Medicine Mar 2021
– notice: The American Society of Tropical Medicine and Hygiene 2021
DBID AAYXX
CITATION
CGR
CUY
CVF
ECM
EIF
NPM
7X8
5PM
DOI 10.4269/ajtmh.20-1219
DatabaseName CrossRef
Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
MEDLINE - Academic
PubMed Central (Full Participant titles)
DatabaseTitle CrossRef
MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
MEDLINE - Academic
DatabaseTitleList
MEDLINE
MEDLINE - Academic


CrossRef
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
Discipline Medicine
EISSN 1476-1645
EndPage 841
ExternalDocumentID PMC7941839
33350371
10_4269_ajtmh_20_1219
Genre Research Support, Non-U.S. Gov't
Systematic Review
Journal Article
GroupedDBID ---
23M
2WC
34G
36B
53G
5GY
5RE
5VS
6J9
AAYXX
ABCQX
ABPPZ
ACGFO
ADBBV
ADTPD
AENEX
AIAGR
ALMA_UNASSIGNED_HOLDINGS
AOIJS
BAWUL
BTFSW
CITATION
DIK
E3Z
EBD
EBS
EJD
EMB
EMOBN
F5P
GX1
H13
HYE
K-O
KQ8
L7B
MV1
OK1
P2P
PQQKQ
RHI
RPM
SV3
TR2
TST
UPT
W8F
WH7
WOQ
WOW
~KM
CGR
CUY
CVF
ECM
EIF
NPM
RHF
7X8
5PM
ID FETCH-LOGICAL-c373t-3bf5e3a2b3cbb403557f5f7182fea4f8fa4d83cce8dc5b1ee241011c60a583723
ISSN 0002-9637
1476-1645
IngestDate Thu Aug 21 18:43:09 EDT 2025
Fri Jul 11 12:05:35 EDT 2025
Mon Jun 30 13:37:26 EDT 2025
Mon Jun 30 13:45:29 EDT 2025
Thu Jan 02 22:55:15 EST 2025
Thu Apr 24 23:11:32 EDT 2025
Tue Jul 01 03:04:14 EDT 2025
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 3
Language English
License https://creativecommons.org/licenses/by-nc/4.0
Open Access statement. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (https://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted use, distribution, and reproduction in any medium for non-commercial purposes, provided the original author and source are credited, a link to the CC License is provided, and changes – if any – are indicated.
LinkModel OpenURL
MergedId FETCHMERGED-LOGICAL-c373t-3bf5e3a2b3cbb403557f5f7182fea4f8fa4d83cce8dc5b1ee241011c60a583723
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
ObjectType-Undefined-3
Authors’ addresses: Debashish Das, Prabin Dahal, Barbara Wanjiru Citarella, Kalynn Kennon, Kasia Stepniewska, and Philippe J. Guerin, Infectious Diseases Data Observatory (IDDO), Centre for Tropical Medicine, University of Oxford, United Kingdom, and Nuffield Department of Medicine, Global Health, University of Oxford, United Kingdom, E-mails: debashish.das@wwarn.org, prabin.dahal@wwarn.org, barbara.citarella@wwarn.org, kalynn.kennon@wwarn.org, kasia.stepniewska@wwarn.org, and philippe.guerin@wwarn.org. Mehul Dhorda, Infectious Diseases Data Observatory (IDDO), Asia-Pacific Regional Centre, Bangkok, Thailand, Nuffield Department of Medicine, Centre for Tropical Medicine and Global Health, University of Oxford, United Kingdom, and Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand, E-mail: mehul.dhorda@wwarn.org. Ingrid Felger, Swiss Tropical and Public Health Institute, Basel, Switzerland, E-mail: ingrid.felger@swisstph.ch. François Chappuis, Division of Tropical and Humanitarian Medicine, Geneva University Hospitals and University of Geneva, Geneva, Switzerland, E-mail: francois.chappuis@hcuge.ch.
Disclosure: All the data used in this review are presented in the main text, either as tables/figures or as Supplemental Files. Raw data used for analysis are presented as Supplemental File 3. The R script used for processing the raw data and generating tables and figures presented in the main text is presented as Supplemental File 4.
Financial support: The WorldWide Antimalarial Resistance Network (WWARN) is funded by the Bill & Melinda Gates Foundation and the ExxonMobil Foundation.
OpenAccessLink https://pubmed.ncbi.nlm.nih.gov/PMC7941839
PMID 33350371
PQID 2528500593
PQPubID 105381
PageCount 6
ParticipantIDs pubmedcentral_primary_oai_pubmedcentral_nih_gov_7941839
proquest_miscellaneous_2472108262
proquest_journals_2560413024
proquest_journals_2528500593
pubmed_primary_33350371
crossref_citationtrail_10_4269_ajtmh_20_1219
crossref_primary_10_4269_ajtmh_20_1219
ProviderPackageCode CITATION
AAYXX
PublicationCentury 2000
PublicationDate 2021-03-01
PublicationDateYYYYMMDD 2021-03-01
PublicationDate_xml – month: 03
  year: 2021
  text: 2021-03-01
  day: 01
PublicationDecade 2020
PublicationPlace United States
PublicationPlace_xml – name: United States
– name: Deerfield, Illinois
PublicationTitle The American journal of tropical medicine and hygiene
PublicationTitleAlternate Am J Trop Med Hyg
PublicationYear 2021
Publisher Institute of Tropical Medicine
The American Society of Tropical Medicine and Hygiene
Publisher_xml – name: Institute of Tropical Medicine
– name: The American Society of Tropical Medicine and Hygiene
SSID ssj0018211
Score 2.3715262
SecondaryResourceType review_article
Snippet Microscopy of stained blood films is essential for the diagnosis of malaria, differentiation of parasite species, and estimation of parasite density performed...
SourceID pubmedcentral
proquest
pubmed
crossref
SourceType Open Access Repository
Aggregation Database
Index Database
Enrichment Source
StartPage 836
SubjectTerms Clinical trials
Clinical Trials as Topic
Drug efficacy
Humans
Malaria
Malaria - diagnosis
Medical diagnosis
Methods
Microscopy
Microscopy - methods
Parasites
Review
Title A Systematic Literature Review of Microscopy Methods Reported in Malaria Clinical Trials
URI https://www.ncbi.nlm.nih.gov/pubmed/33350371
https://www.proquest.com/docview/2528500593
https://www.proquest.com/docview/2560413024
https://www.proquest.com/docview/2472108262
https://pubmed.ncbi.nlm.nih.gov/PMC7941839
Volume 104
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3db9MwELfKkBAviG86BjIS4qUEGn8k2WPVbUyoHS-p6FvkuPaSCdKpTR-2f4d_lHMc52MwBLxEVWxHlu_n81199zuE3kapINE40l5wKCKPMSE8wULmaQAMEVoJPzT5zvOz4HTBPi_5cjD40Yla2pXpB3n927yS_5EqvAO5mizZf5Bs81F4Ab9BvvAECcPzr2Q8qQnHK9bVWUOQ7NjyTXSLibczmSdXo3lVK3pbm9zKcC6N5gIc21yMpi4_Mq5m3bVY4zb1pOjyTJSb9WU1xN3OV9cQ2dU56IoGLEc2XQyUmqnZtM3a95mwtZM3Is0bfB5l4ApX1uxcZW3E4jQvxcZEaXUuSEZfRXGRb3bdfy1IJ2zLerLdSIjYTXjeDSdoFTboCHsmK6ujWRh44OXxnhK3RYxrtNKOSo4swcrNo8Kk8Jpz8KL8bu6kPN-p7h4l99mX5GQxmyXx8TK-g-4S8EVMmYxPyyaOCPwz33c-lpmpJXI1n__Y-3jf8PnFm7kZlNuxcuKH6EHtnuCJxdojNFDFY3TPrdgTtJzgFnK4hRy2kMNrjVvI4Rpy2EEO5wWuIYcd5LCF3FO0ODmOp6deXZzDkzSkpUdTzRUVJKUyTdkYzNZQcw2WDoENznSkBVtFVEoVrSRPfaXAVISzRAZjwSMaEvoM7RXrQr1AmDNfs7GQZgBTKj3kK8E1hTVbaS4lGaL3buUSWTPXmwIq3xLwYM1CJ9VCJwT8WRg0RO-a7peWsuW2jgdODEm9f7YJ4STiVaHLW5qDsbH7CBuiN00zqGRzzyYKtd5BHxYSH0zrAGb-3Aq1mQillBuWzCEKe-JuOhi6935LkWcV7TucnMad2f_ztF6i--1-O0B75WanXoHdXKavK9z-BDpmyv4
linkProvider Geneva Foundation for Medical Education and Research
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=A+Systematic+Literature+Review+of+Microscopy+Methods+Reported+in+Malaria+Clinical+Trials&rft.jtitle=The+American+journal+of+tropical+medicine+and+hygiene&rft.au=Das%2C+Debashish&rft.au=Dahal%2C+Prabin&rft.au=Dhorda%2C+Mehul&rft.au=Citarella%2C+Barbara+Wanjiru&rft.date=2021-03-01&rft.pub=Institute+of+Tropical+Medicine&rft.issn=0002-9637&rft.eissn=1476-1645&rft.volume=104&rft.issue=3&rft.spage=836&rft_id=info:doi/10.4269%2Fajtmh.20-1219&rft.externalDBID=NO_FULL_TEXT
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0002-9637&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0002-9637&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0002-9637&client=summon