Optimization of the refolding of recombinant human granulocyte-macrophage colony-stimulating factor immobilized on affinity sorbent

• Published in: Applied biochemistry and microbiology Vol. 50; no. 8; pp. 773 - 779
• Main Authors: Kosobokova, E. N, Skrypnik, K. A, Pinyugina, M. V, Shcherbakov, A. I, Kosorukov, V. S
• Format: Journal Article
• Language: English
• Published: Moscow Springer-Verlag 01.12.2014; Pleiades Publishing; Springer Nature B.V
• Subjects: bioactive properties; Biochemistry; Biomedical and Life Sciences; E coli; Escherichia coli; granulocyte-macrophage colony-stimulating factor; humans; Life Sciences; Medical Microbiology; Microbiology; Proteins; Sorbents; Technology of Biopreparations; washing; GM-CSF; on-column refolding; His-domain

A method for the production and purification of biologically active recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF), expressed in Escherichia coli, has been developed. Washing of an inclusion body fraction which allows the removal of numerous ballast proteins and on-col...