Infectious in vivo Transcripts from a Full-length Clone of Soybean mosaic virus Strain G5H

• Published in: The plant pathology journal Vol. 25; no. 1; pp. 54 - 61
• Main Authors: Seo, J.K. (Seoul National University, Seoul, Republic of Korea), Lee, H.G. (Seoul National University, Seoul, Republic of Korea), Choi, H.S. (National Institute of Agricultural Science and Technology, RDA, Suwon, Republic of Korea), Lee, S.H. (National Institute of Agricultural Science and Technology, RDA, Suwon, Republic of Korea), Kim, K.H. (Seoul National University, Seoul, Republic of Korea), E-mail: kookkim@snu.ac.kr
• Format: Journal Article
• Language: English
• Published: 한국식물병리학회 01.03.2009
• Subjects: DNA-based vector; Infectious clone; POTYVIRUS DEL MOSAICO DE LA SOJA; POTYVIRUS MOSAIQUE SOJA; SOYBEAN MOSAIC POTYVIRUS; 농학; Infectious clone; DNA-based vector; SMV
• ISSN: 1598-2254; 2093-9280
• DOI: 10.5423/PPJ.2009.25.1.054

An infectious full-length clone of Soybean mosaic virus (SMV) strain G5H was constructed under the control of the cauliflower mosaic virus 35S promoter. The cloned SMV G5H established infections upon simple rubinoculation of soybean leaves with intact plasmid DNA. We demonstrated that this SMV G5H infectious DNA clone caused typical characteristic symptoms and virulence of SMV strain G5H in twelve tested soybean cultivars. Soybean cultivars Lee74, Somyungkong and Sowonkong developed systemic mosaic symptom while Kwanggyo, Taekwangkong, Hwangkeumkong and Geumjeongkong-1 showed systemic necrosis. In contrast, Geumjeongkong-2, Jinpumkong-2, L29, V94-5152 and Ogden showed resistant response against SMV-G5H infection. We also determined full-length sequence of cloned SMV-G5H. The phyogenetic analyses reveal that SMV-G5H is most closely related to SMV-G5, and support that SMV-G5H might be derived from SMV-G5 by recombination rather than mutation.