On the Mechanisms of Hypohalous Acid Formation and Electrophilic Halogenation by Non‐Native Halogenases

• Published in: Angewandte Chemie International Edition Vol. 63; no. 24; pp. e202403858 - n/a
• Main Authors: Prakinee, Kridsadakorn, Lawan, Narin, Phintha, Aisaraphon, Visitsatthawong, Surawit, Chitnumsub, Penchit, Jitkaroon, Watcharapa, Chaiyen, Pimchai
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 10.06.2024
• Edition: International ed. in English
• Subjects: De novo function; Electrophilic halogenation; Enzyme catalysis; Enzymes; Flavin; Flavin reductase; Flavin-dependent enzymes; Flavins - chemistry; Flavins - metabolism; Fluorimetry; Fluorometry; Halides; Halogenation; Hydrogen peroxide; Hydrogen Peroxide - chemistry; Hydrogen Peroxide - metabolism; Hydrolases - chemistry; Hydrolases - metabolism; Kinetics; Mixed Function Oxygenases - chemistry; Mixed Function Oxygenases - metabolism; Mutagenesis; Organic compounds; Oxidoreductases - chemistry; Oxidoreductases - metabolism; Reaction mechanisms; Reductases; Spectrophotometry; Structural analysis; Substrates; Flavin-dependent enzymes; De novo function; Enzyme catalysis; Reaction mechanisms; Electrophilic halogenation

Enzymatic electrophilic halogenation is a mild tool for functionalization of diverse organic compounds. Only a few groups of native halogenases are capable of catalyzing such a reaction. In this study, we used a mechanism‐guided strategy to discover the electrophilic halogenation activity catalyzed...