Tailor‐Made Polyhydroxyalkanoates by Reconstructing Pseudomonas Entomophila

• Published in: Advanced materials (Weinheim) Vol. 33; no. 41; pp. e2102766 - n/a
• Main Authors: Li, Mengyi, Ma, Yueyuan, Zhang, Xu, Zhang, Lizhan, Chen, Xinyu, Ye, Jian‐Wen, Chen, Guo‐Qiang
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 01.10.2021
• Subjects: Acyl Coenzyme A - metabolism; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Carbon; Copolymers; Fatty acids; Fatty Acids - metabolism; flux tuning; Glucose - metabolism; Hydroxybutyrates - chemistry; Hydroxybutyrates - metabolism; Mechanical properties; metabolic engineering; Microorganisms; Molecular chains; Monomers; Multigene Family; Oxidation; Oxidation-Reduction; Plasmids - genetics; Plasmids - metabolism; Polyesters - chemistry; Polyesters - metabolism; Polyhydroxyalkanoates; Polyhydroxyalkanoates - chemistry; Polyhydroxyalkanoates - metabolism; Pseudomonas; Pseudomonas - genetics; Pseudomonas - metabolism; Pseudomonas entomophila; SCL‐co‐LCL PHA copolymer; SCL‐co‐MCL PHA copolymer; Synthesis; synthetic biology; Thermodynamic properties; Weight; Pseudomonas entomophila; flux tuning; synthetic biology; SCL-co-LCL PHA copolymer; metabolic engineering; polyhydroxyalkanoates; SCL-co-MCL PHA copolymer
• ISSN: 0935-9648; 1521-4095
• DOI: 10.1002/adma.202102766

Microbial polyhydroxyalkanoates (PHA) containing short‐ and medium/long‐chain‐length monomers, abbreviated as SCL‐co‐MCL/LCL PHAs, generate suitable thermal and mechanical properties. However, SCL‐co‐MCL/LCL PHAs with carbon chain longer than nine are difficult to synthesize due to the low specificity of PHA synthase PhaC and the lack of either SCL‐ or MCL/LCL monomer precursor fluxes. This study succeeds in reprogramming a β‐oxidation weakened Pseudomonas entomophila containing synthesis pathways of SCL 3‐hydroxybutyryl‐CoA (3HB) from glucose and MCL/LCL 3‐hydroxyalkanoyl‐CoA from fatty acids with carbon chain lengths from 9 to 18, respectively, that are polymerized under a low specificity PhaC61‐3 to form P(3HB‐co‐MCL/LCL 3HA) copolymers. Through rational flux‐tuning approaches, the optimized recombinant P. entomophila accumulates 55 wt% poly‐3‐hydroxybutyrate in 8.4 g L−1 cell dry weight. Combined with weakened β‐oxidation, a series of novel P(3HB‐co‐MCL/LCL 3HA) copolymers with over 60 wt% PHA in 9 g L−1 cell dry weight have been synthesized for the first time. P. entomophila has become a high‐performing platform to generate tailor‐made new SCL‐co‐MCL/LCL PHAs. A high‐performing bacterial platform for customized SCL‐co‐MCL/LCL PHA synthesis is developed via rational fine‐tuning approaches, based on which, a series of P(3HB‐co‐MCL/LCL 3HA) copolymers are generated with 3HA ranging from 9 to 18 carbon atoms. The PHA copolymer family is thus further expanded, generating various novel material properties and broader prospects for value‐added applications.