Synthetic and analytical strategies for the quantification of phenyl‐γ‐valerolactone conjugated metabolites in human urine

• Published in: Molecular nutrition & food research Vol. 61; no. 9; pp. 1700077 - n/a
• Main Authors: Brindani, Nicoletta, Mena, Pedro, Calani, Luca, Benzie, Iris, Choi, Siu‐Wai, Brighenti, Furio, Zanardi, Franca, Curti, Claudio, Del Rio, Daniele
• Format: Journal Article
• Language: English
• Published: Germany 01.09.2017
• Subjects: Adult; bioactive properties; bioavailability; chromatography; Chromatography, High Pressure Liquid; Colon - metabolism; Colonic metabolites; detection limit; excretion; flavanols; Flavan‐3‐ol; Flavonoids - metabolism; Green tea; Humans; intestinal microorganisms; Lactones - analysis; Lactones - chemical synthesis; Limit of Detection; metabolism; metabolites; Middle Aged; Phenolic compounds; proanthocyanidins; Spectrometry, Mass, Electrospray Ionization; Stereoselective synthesis; sulfates; Tandem Mass Spectrometry; Tea; urine; Colonic metabolites; Flavan-3-ol; Phenolic compounds; Green tea; Stereoselective synthesis

Scope The contribution of the gut microbiota to the metabolism of catechins and proanthocyanidins remains unclear. Phenyl‐γ‐valerolactones have been identified as the most representative metabolites of these dietary flavan‐3‐ols, but their accurate quantification has posed problems because of a lack...