Methyl and Isopropyl N‐Methylanthranilates Affect Primary Macrophage Function – An Insight into the Possible Immunomodulatory Mode of Action

• Published in: Chemistry & biodiversity Vol. 19; no. 1; pp. e202100724 - n/a
• Main Authors: Miltojević, Ana B., Mitić, Katarina V., Stojanović, Nikola M., Randjelović, Pavle J., Radulović, Niko S.
• Format: Journal Article
• Language: English
• Published: Switzerland Wiley Subscription Services, Inc 01.01.2022
• Subjects: Animals; anti-inflammatory activity; Anti-Inflammatory Agents - chemistry; Anti-Inflammatory Agents - metabolism; Anti-Inflammatory Agents - pharmacology; Catalase; Catalase - antagonists & inhibitors; Catalase - metabolism; Cell membranes; Cell Survival - drug effects; Cells, Cultured; Enzymatic activity; Enzyme activity; Esters; Immunomodulation; immunomodulatory potential; Inflammation; Inflammatory response; macrophage function; Macrophages; Macrophages - cytology; Macrophages - immunology; Macrophages - metabolism; Male; Mode of action; N-methylanthranilic acid esters; Natural products; ortho-Aminobenzoates - chemistry; ortho-Aminobenzoates - metabolism; ortho-Aminobenzoates - pharmacology; oxidoreductases; Peroxidase; Peroxidase - metabolism; Phagocytes; Phagocytosis - drug effects; Rats; Rats, Wistar; Xanthine oxidase; Xanthine Oxidase - antagonists & inhibitors; Xanthine Oxidase - metabolism; oxidoreductases; macrophage function; N-methylanthranilic acid esters; anti-inflammatory activity; immunomodulatory potential
• ISSN: 1612-1872; 1612-1880
• DOI: 10.1002/cbdv.202100724

To complement the knowledge on the anti‐inflammatory activity of methyl and isopropyl N‐methylanthranilates, two natural products with panacea‐like properties, we investigated their effects on thioglycolate‐elicited macrophages by evaluating macrophage ability to metabolize MTT, macrophage membrane function, and macrophage myeloperoxidase and phagocytic activities. Moreover, two additional aspects of the inflammatory response of these compounds, their inhibitory activity on xanthine oxidase and catalase, were studied. It was found that these two compounds regulate elicited macrophage functions, most probably by interfering with the function of cell membranes and changing the reducing cellular capacity or enzyme activity of macrophages. Nonetheless, no significant inhibitory action either towards xanthine oxidase or catalase was found, suggesting that the inhibition of these enzymes is not involved in the anti‐inflammatory mode of action of these two esters.