Achieving Biofunctional Micropatterns via Protein‐Based Aqueous Photoresists with Tailored Functionalities

• Published in: Small (Weinheim an der Bergstrasse, Germany) Vol. 21; no. 19; pp. e2411900 - n/a
• Main Authors: Wang, Jiaqi, Li, Zishun, Wang, Min, Shang, Hongpeng, Ding, Jie, Zheng, Xiaorui, Guo, Chengchen
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 01.05.2025
• Subjects: Animals; aqueous photoresist; Biocompatibility; Biocompatible Materials - chemistry; biofunctional micropattern; Biological activity; Biosensors; Biosynthesis; chemically modified protein; Conjugation; diagnostics; Fibroins - chemistry; High temperature; Micropatterning; Nucleic acids; Photolithography; Photoresists; Proteins; Proteins - chemistry; Reagents; Silk fibroin; Water - chemistry; Silk fibroin; diagnostics; aqueous photoresist; biofunctional micropattern; chemically modified protein
• ISSN: 1613-6810; 1613-6829; 1613-6829
• DOI: 10.1002/smll.202411900

Photolithography is the most widely used micropatterning technique at the micro‐ and nanoscale in device fabrication. However, traditional photoresists used in photolithography are typically nonaqueous‐based toxic substances that require harsh conditions for processing, limiting the development of biofunctional and biocompatible micropatterns. In this study, a protein‐based aqueous photoresist derived from chemically modified silk fibroin named SAMA, capable of achieving high‐resolution micropatterning (<1.2 µm) while retaining good biocompatibility, is presented. The entire fabrication process, including spin‐coating, development, and lift‐off, employs solely SAMA and water, eliminating the need for toxic reagents and elevated temperature. Notably, the SAMA photoresist allows covalent conjugation of biofunctional molecules, such as enzymes and nucleic acids, while preserving their bioactivity during micropatterning. This innovative approach enables the high‐throughput generation of bioactive micropatterns for various applications such as biosynthesis, diagnostics, and biosensors. This study introduces a protein‐based aqueous photoresist derived from chemically modified silk fibroin that employs an entirely water‐based process for achieving high‐resolution micropatterning (<1.2 µm) with excellent biocompatibility. The conjugation of biofunctional molecules to the photoresist further allows the efficient and high‐throughput fabrication of multiplexed biofunctional micropatterns, with potential applications in biosynthesis, diagnostics, and biosensors.