Free Ig light chains interact with sphingomyelin and are found on the surface of myeloma plasma cells in an aggregated form

• Published in: The Journal of immunology (1950) Vol. 185; no. 7; pp. 4179 - 4188
• Main Authors: Hutchinson, Andrew T, Ramsland, Paul A, Jones, Darren R, Agostino, Mark, Lund, Maria E, Jennings, Cameron V, Bockhorni, Vanessa, Yuriev, Elizabeth, Edmundson, Allen B, Raison, Robert L
• Format: Journal Article
• Language: English
• Published: United States 01.10.2010
• Subjects: Blotting, Western; Cell Line, Tumor; Cell Membrane - chemistry; Cell Membrane - immunology; Cell Membrane - metabolism; Cell Separation; Electrophoresis, Polyacrylamide Gel; Enzyme-Linked Immunosorbent Assay; Flow Cytometry; Humans; Immunoglobulin Light Chains - metabolism; Multiple Myeloma - metabolism; Multiple Myeloma - pathology; Multiprotein Complexes; Plasma Cells - metabolism; Plasma Cells - pathology; Receptor Aggregation - physiology; Sphingomyelins - metabolism; Transfection

Free κ L chains (FκLCs) are expressed on the surface of myeloma cells and are being assessed as a therapeutic target for the treatment of multiple myeloma. Despite its clinical potential, the mechanism by which FκLCs interact with membranes remains unresolved. In this study, we show that FκLCs assoc...