Tylosin in anaerobic reactors: degradation kinetics, effects on methane production and on the microbial community

• Published in: Biodegradation (Dordrecht) Vol. 33; no. 3; pp. 283 - 300
• Main Authors: Paranhos, Aline Gomes de Oliveira, Pereira, Andressa Rezende, da Fonseca, Yasmim Arantes, de Queiroz Silva, Silvana, de Aquino, Sérgio Francisco
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.06.2022; Springer; Springer Nature B.V
• Subjects: Acetates; Acetic acid; Acetogenesis; Acid production; Agricultural wastes; Alternative energy sources; Anaerobic digestion; Anaerobic microorganisms; Anaerobic systems; Anaerobic treatment; Anaerobiosis; Animal wastes; Antibiotics; Antimicrobial agents; Aquatic Pollution; Archaea; Biodegradation; Biodegradation, Environmental; Biogas; Biomedical and Life Sciences; Bioreactors; Bioreactors - microbiology; Degradation; Geochemistry; Health aspects; Kinetics; Life Sciences; Metagenomics; Methane; Methane - metabolism; Methanogenesis; Methanogenic bacteria; Microbiology; Microbiota; Microorganisms; Original Paper; Oxytetracycline; Poultry; Reactors; Soil Science & Conservation; Terrestrial Pollution; Tylosin; Tylosin - pharmacology; Waste Management/Waste Technology; Waste Water Technology; Water Management; Water Pollution Control; Veterinary antibiotic; Metagenomics; Acetogenesis; Methanogenesis; Poultry litter
• DOI: 10.1007/s10532-022-09980-3
• ISSN: 0923-9820

Tylosin eliminated in animal waste, during therapeutic treatment, can be efficiently removed in anaerobic systems. The present study investigated the influence of tylosin concentration and assessed its degradation kinetics and the microorganisms involved in each stage of its anaerobic digestion (hydrolysis/acidogenesis; acetogenesis; methanogenesis). The results showed a stimulating effect on methane production with increasing tylosin concentration in the poultry litter up to 80 mg kg −1 tylosin (232.9 NL CH 4  kg SV −1 ). As for tylosin degradation, greater removal of antibiotics was observed in the methanogenic phase (88%), followed by acetogenic (84%) and hydrolytic/acidogenic (76%) phases. The higher rate of tylosin degradation obtained in the methanogenic step, is mainly related to the co-metabolic effect exerted by the presence of acetate and its degradation by acetoclastic methanogens. Indeed, metagenomic analyses suggested a syntrophic action between archaea of ​​the genus Methanobacterium , and bacteria such as Clostridium and Flexilinea , which seemed decisive for tylosin degradation.