Real-time analysis of dynamic compartmentalized GSH redox shifts and H2O2 availability in undifferentiated and differentiated cells

• Published in: Biochimica et biophysica acta. General subjects Vol. 1867; no. 5; p. 130321
• Main Authors: Davies, Brandon M., Katayama, Jenna K., Monsivais, Joshua E., Adams, James R., Dilts, Miriam E., Eberting, Arielle L., Hansen, Jason M.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.05.2023
• Subjects: antioxidants; cell differentiation; confocal microscopy; cytosol; Glutathione; Hydrogen peroxide; mitochondria; Neurogenesis; Nuclear factor erythroid-2-related factor 2; oxidants; oxidation; Redox state; Redox-sensitive green fluorescent protein; NADPH; Hydrogen peroxide; Eh; D3T; DMSO; DTT; H2O2; MTT; Neurogenesis; roGFP; OCT4; DMEM; NLS; MLS; Nrf2; HMOX1; Nuclear factor erythroid-2-related factor 2; GD; GSH; RT-qPCR; HPLC; Glutathione; Redox state; GSHtot; GSSG; NQO1; Orp1; Pr-SSG; Grx1; DAPI; Redox-sensitive green fluorescent protein; CMV; ER; ef1α; PCA; RA; SDS-PAGE; GCLC; HEPES; PBST; ROS; DCF-DA; NES; BA

Glutathione (GSH) is the most abundant, small biothiol antioxidant. GSH redox state (Eh) supports developmental processes, yet with disrupted GSH Eh, poor developmental outcomes may occur. The role of subcellular, compartmentalized redox environments in the context of redox regulation of differentia...