Rewiring bacterial two-component systems by modular DNA-binding domain swapping

• Published in: Nature chemical biology Vol. 15; no. 7; pp. 690 - 698
• Main Authors: Schmidl, Sebastian R., Ekness, Felix, Sofjan, Katri, Daeffler, Kristina N.-M., Brink, Kathryn R., Landry, Brian P., Gerhardt, Karl P., Dyulgyarov, Nikola, Sheth, Ravi U., Tabor, Jeffrey J.
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.07.2019; Nature Publishing Group
• Subjects: 631/326/41; 631/337/572; 631/92/469; 631/92/552; Binding; Biochemical Engineering; Biochemistry; Bioorganic Chemistry; Cell Biology; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Coding; Deoxyribonucleic acid; DNA; DNA, Bacterial - genetics; DNA, Bacterial - metabolism; Domains; E coli; Escherichia coli - genetics; Escherichia coli - metabolism; Genetic code; Genetic Engineering; Modular systems; pH sensors; Promoters; Rewiring; Sensors; Shewanella - genetics; Shewanella - metabolism; Signal transduction; Transduction

Two-component systems (TCSs) are the largest family of multi-step signal transduction pathways and valuable sensors for synthetic biology. However, most TCSs remain uncharacterized or difficult to harness for applications. Major challenges are that many TCS output promoters are unknown, subject to c...