Monitoring Real-time Temperature Dynamics of a Short RNA Hairpin Using Förster Resonance Energy Transfer and Circular Dichroism

• Published in: Bio-protocol Vol. 11; no. 6; p. e3950
• Main Authors: Balcerowicz, Martin, Di Antonio, Marco, Chung, Betty
• Format: Journal Article
• Language: English
• Published: United States Bio-Protocol 20.03.2021; Bio-protocol LLC
• Subjects: Biology; Methods; Temperature; FRET; RNA structure; CD spectroscopy

RNA secondary structures are highly dynamic and subject to prompt changes in response to the environment. Temperature in particular has a strong impact on RNA structural conformation, and temperature-sensitive RNA hairpin structures have been exploited by multiple organisms to modify the rate of tra...