Post-synthesis of covalent organic frameworks with dual-hydrophilic groups for specific capture of serum exosomes

• Published in: Journal of Chromatography A Vol. 1679; p. 463406
• Main Authors: Hua, Shuwen, Feng, Quanshou, Xie, Zehu, Mao, Haijiao, Zhou, Yuping, Yan, Yinghua, Ding, Chuan-Fan
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 30.08.2022
• Subjects: Amino; biomarkers; blood serum; carcinoma; Covalent organic frameworks; cysteine; detection limit; Exosomes; glutathione; glycopeptides; Glycopeptides enrichment; glycosylation; humans; hydrophilicity; kidney neoplasms; liquid chromatography; MALDI-TOF MS; physiological state; tandem mass spectrometry; Covalent organic frameworks; MALDI-TOF MS; Amino; Exosomes; Glycopeptides enrichment

•A hydrophilic COF was prepared via the post-synthesis modification strategy.•COF-S@Au@GC was used for selective enrichment glycosylated exosomes in serum.•182 glycopeptides were detected from serum after treated with COF-S@Au@GC. Exosomes can reflect the physiological state of parent cells and are...