Column cation-exchange separation of melanin-related metabolites in urine from cases of melanoma

We describe recent developments in the use of a stable free radical, diphenylpicrylhydrazyl, as a colorimetric detector of reducing metabolites in melanoma urines. A procedure for buffering aqueous/solvent reagent solutions is presented, and examples of the baseline stability achieved for reference...

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Published inClinical chemistry (Baltimore, Md.) Vol. 23; no. 8; pp. 1397 - 1401
Main Authors Banda, PW, Sherry, AE, Blois, MS
Format Journal Article
LanguageEnglish
Published England Am Assoc Clin Chem 01.08.1977
Subjects
Animals
Chromatography, Ion Exchange - methods
Cricetinae
Cysteinyldopa - urine
Humans
Hydrazines
Melanins - urine
Melanoma - urine
Picrates
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Abstract We describe recent developments in the use of a stable free radical, diphenylpicrylhydrazyl, as a colorimetric detector of reducing metabolites in melanoma urines. A procedure for buffering aqueous/solvent reagent solutions is presented, and examples of the baseline stability achieved for reference chromatograms and urine samples are provided. Chromatograms of phaeomelanin precursors and of an extract of a highly pigmented hamster melanoma are also presented. Identities are tentatively assigned for some of the chromatographic peaks that have previously been correlated with disease, including isomers of cysteinyldopa, and observations of new pigment- and tumor-related metabolites in the chromatograms are noted.
AbstractList We describe recent developments in the use of a stable free radical, diphenylpicrylhydrazyl, as a colorimetric detector of reducing metabolites in melanoma urines. A procedure for buffering aqueous/solvent reagent solutions is presented, and examples of the baseline stability achieved for reference chromatograms and urine samples are provided. Chromatograms of phaeomelanin precursors and of an extract of a highly pigmented hamster melanoma are also presented. Identities are tentatively assigned for some of the chromatographic peaks that have previously been correlated with disease, including isomers of cysteinyldopa, and observations of new pigment- and tumor-related metabolites in the chromatograms are noted.
Abstract We describe recent developments in the use of a stable free radical, diphenylpicrylhydrazyl, as a colorimetric detector of reducing metabolites in melanoma urines. A procedure for buffering aqueous/solvent reagent solutions is presented, and examples of the baseline stability achieved for reference chromatograms and urine samples are provided. Chromatograms of phaeomelanin precursors and of an extract of a highly pigmented hamster melanoma are also presented. Identities are tentatively assigned for some of the chromatographic peaks that have previously been correlated with disease, including isomers of cysteinyldopa, and observations of new pigment- and tumor-related metabolites in the chromatograms are noted.
Author Blois, MS
Sherry, AE
Banda, PW
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Snippet We describe recent developments in the use of a stable free radical, diphenylpicrylhydrazyl, as a colorimetric detector of reducing metabolites in melanoma...
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SubjectTerms Animals
Chromatography, Ion Exchange - methods
Cricetinae
Cysteinyldopa - urine
Humans
Hydrazines
Melanins - urine
Melanoma - urine
Picrates
Title Column cation-exchange separation of melanin-related metabolites in urine from cases of melanoma
URI http://www.clinchem.org/cgi/content/abstract/23/8/1397
https://www.ncbi.nlm.nih.gov/pubmed/872403
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