High-throughput genome-wide phenotypic screening via immunomagnetic cell sorting

• Published in: Nature biomedical engineering Vol. 3; no. 10; pp. 796 - 805
• Main Authors: Mair, Barbara, Aldridge, Peter M., Atwal, Randy S., Philpott, David, Zhang, Meng, Masud, Sanna N., Labib, Mahmoud, Tong, Amy H. Y., Sargent, Edward H., Angers, Stéphane, Moffat, Jason, Kelley, Shana O.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.10.2019; Nature Publishing Group
• Subjects: 45; 45/23; 631/1647/2163; 631/1647/2217; 631/1647/277; Biomedical and Life Sciences; Biomedical Engineering/Biotechnology; Biomedicine; Cancer immunotherapy; CD47 Antigen - metabolism; Cell Line, Tumor; Cell surface; Cell viability; CRISPR; CRISPR-Cas Systems; Flow Cytometry; Fluorescence; Gene Editing; Genetic screening; Genome; Genomes; Glutamine; High-Throughput Screening Assays - methods; Humans; Immunomagnetic Separation - methods; Immunomodulation; Immunotherapy; Lab-On-A-Chip Devices; Microfluidics; Modulators; Neoplasms - therapy; Oncology; Phagocytosis; Phenotype; Phenotypes; Regulators; Screening

Genome-scale functional genetic screens are used to identify key genetic regulators of a phenotype of interest. However, the identification of genetic modifications that lead to a phenotypic change requires sorting large numbers of cells, which increases operational times and costs and limits cell v...