Biochemical and Morphological Parameters of Inbred/Outbred Lines and DBCB Tetrahybrid Mouse in High-Sugar In Vivo Model of Metabolic Syndrome

• Published in: Bulletin of experimental biology and medicine Vol. 166; no. 1; pp. 96 - 101
• Main Authors: Apryatin, S. A., Mzhel’skaya, K. V., Trusov, N. V., Balakina, A. S., Soto, Kh. S., Beketova, N. A., Kosheleva, O. V., Gmoshinskii, I. V., Nikityuk, D. B.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.11.2018; Springer Nature B.V
• Subjects: Animals; Biomedical and Life Sciences; Biomedicine; Cell Biology; Disease Models, Animal; Fatty liver; Fatty Liver - metabolism; Fatty Liver - pathology; Female; Hypercholesterolemia; Inbreeding; Internal Medicine; Laboratory Medicine; Liver; Liver - metabolism; Liver - pathology; Liver diseases; Male; Metabolic syndrome; Metabolic Syndrome - metabolism; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mice, Inbred DBA; Morphology; Morphology and Pathomorphology; Pathology; Proteomics; Proteomics - methods; Rodents; Steatosis; Sucrose; Sucrose - adverse effects; Sucrose - metabolism; Sugar; Vitamin E; Vitamins; metabolic syndrome; tetrahybrid; in vivo model; sucrose; mice
• DOI: 10.1007/s10517-018-4296-2
• ISSN: 0007-4888

Integral, biochemical, and morphological parameters and concentrations of vitamins, particularly lipid soluble vitamins, were analyzed in female mice of inbred DBA/2J line, outbred ICR-1 (CD-1) line, and DBCB tetrahybrid mice on the in vivo model of metabolic syndrome induced by consumption of 30% sucrose for 2 days. In contrast to inbred and outbred lines, DBCB tetrahybrid mice developed abdominal obesity, hypercholesterolemia, and pronounced morphological picture of fatty liver disease. The lipid-coupled transport of vitamin E to the liver is also enhanced in these animals, which compensated decreased supply of vitamin E to the liver under conditions of high-sugar ration. The observed interstrain differences can be related to genetic features of the used mouse lines and DBCB tetrahybrid mice and require further genomic, transcriptomic, proteomic, and morphological studies. The results of the study based on the in vivo model of metabolic syndrome allow identifying the key biomarkers for complex diagnostics and prognosis of metabolic syndrome complications, such as nonalcoholic steatosis of the liver.