A mass spectrometry-based proteome map of drug action in lung cancer cell lines

• Published in: Nature chemical biology Vol. 16; no. 10; pp. 1111 - 1119
• Main Authors: Ruprecht, Benjamin, Di Bernardo, Julie, Wang, Zhao, Mo, Xuan, Ursu, Oleg, Christopher, Matthew, Fernandez, Rafael B., Zheng, Li, Dill, Brian D., Wang, Huijun, Xu, Yuting, Liaw, Andy, Mortison, Jonathan D., Siriwardana, Nirodhini, Andresen, Brian, Glick, Meir, Tata, James R., Kutilek, Victoria, Cornella-Taracido, Ivan, Chi, An
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.10.2020; Nature Publishing Group
• Subjects: 631/154; 631/154/1435; 631/553; Antineoplastic Agents - pharmacology; Autophagy; Biochemical Engineering; Biochemistry; Biodegradation; Bioorganic Chemistry; Biotechnology; Cell Biology; Cell Line, Tumor; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Deconvolution; Drugs; Gene Expression Regulation, Neoplastic - drug effects; Gene Expression Regulation, Neoplastic - physiology; Humans; Lung cancer; Lung Neoplasms - metabolism; Mass Spectrometry; Mass spectroscopy; Mitochondria; Perturbation; Phagocytosis; Proteins; Proteome; Proteomes; Proteomics; Proteomics - methods; Robustness; Scientific imaging; Spectroscopy; Target recognition; Tumor cell lines; Workflow

Mass spectrometry-based discovery proteomics is an essential tool for the proximal readout of cellular drug action. Here, we apply a robust proteomic workflow to rapidly profile the proteomes of five lung cancer cell lines in response to more than 50 drugs. Integration of millions of quantitative pr...