mTORC1 activation contributes to autophagy inhibition via its recruitment to lysosomes and consequent lysosomal dysfunction in cadmium-exposed rat proximal tubular cells

• Published in: Journal of inorganic biochemistry Vol. 212; p. 111231
• Main Authors: Lian, Cai-Yu, Yang, Heng, Zhai, Zhen-Zhen, Li, Zi-Fa, Han, Dian-Gang, Wang, Lin
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.11.2020
• Subjects: Autophagy; Cadmium; Kidney; Lysosome; mTORC1; rPT cells; p70S6K; DMSO; Lysosome; rPT cells; PFA; LTR; Autophagy; RIPA; Kidney; 4EBP1; LAMP-2; V-ATPases; DQ-BSA-Green; BCA; Cadmium; Rapa; TFE3; TFEB; DAPI; PVDF; SDS-PAGE; ULK1/ULK2; rPT; RFP-GFP-LC3; ROS; mTORC2; mTORC1

Autophagy dysregulation is implicated in cadmium (Cd)-induced nephrotoxicity. The mammalian target of rapamycin complex 1 (mTORC1) is a negative regulator of autophagy, but its role in Cd-induced autophagy inhibition and possible regulatory mechanisms remains poorly understood. In the present study,...