Phenotypic and genetic variations in crown-gall tumour cells of tobacco

Phenotypic and genetic variations of tumour cells were analysed both quantitatively and qualitatively in clones and subclones of a crown-gall strain. Thus, growth rates, grafting tests, octopine synthesis, estimations of the T-DNA contents, modifications in the numbers, and structures of chromosomes...

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Bibliographic Details
Published inTheoretical and applied genetics Vol. 74; no. 2; pp. 253 - 260
Main Authors Mouras, A, Negrutiu, I, Dessaux, Y
Format Journal Article
LanguageEnglish
Published Germany 01.06.1987
Subjects
Agrobacterium tumefaciens
genetic variation
neoplasms
Nicotiana tabacum
phenotype
plasmids
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Summary:Phenotypic and genetic variations of tumour cells were analysed both quantitatively and qualitatively in clones and subclones of a crown-gall strain. Thus, growth rates, grafting tests, octopine synthesis, estimations of the T-DNA contents, modifications in the numbers, and structures of chromosomes were examined. Phenotypic variations are closely associated with genetic changes, including variation in chromosome number (which is shown to be non-specific to the tumoral state) and, above all, variation in the copy-number of T-DNA sequences per cell, and structural rearrangements of chromosomes. Such rearrangements are characterized by specific marker chromosomes in the tumour cells and they correlate with the degree of oncogenicity of the cells.
ISSN:0040-5752
1432-2242
DOI:10.1007/BF00289977