Real-time qRT-PCR analysis of EGF receptor in cumulus-oocyte complexes recovered by laparoscopy in hormonally treated goats

• Published in: Zygote (Cambridge) Vol. 19; no. 2; pp. 127 - 136
• Main Authors: Almeida, K.C., Pereira, A.F., Alcântara Neto, A.S., Avelar, S.R.G., Bertolini, L.R., Bertolini, M., Freitas, V.J.F., Melo, L.M.
• Format: Journal Article
• Language: English
• Published: Cambridge, UK Cambridge University Press 01.05.2011
• Subjects: Animals; Cumulus Cells - metabolism; Embryos; Female; Follicle Stimulating Hormone - metabolism; Goats; In vitro fertilization; Investigations; Laparoscopy; Oocyte Retrieval; Oocytes - metabolism; Receptor, Epidermal Growth Factor - genetics; Receptor, Epidermal Growth Factor - metabolism; Reverse Transcriptase Polymerase Chain Reaction - methods; Signal transduction; Technological change; EGF receptor; Goat; Real-time qRT-PCR; Oocyte; Cumulus cells

Ovarian stimulation with exogenous follicle stimulating hormone (FSH) has been used to increase the number of viable oocytes for laparoscopic oocyte recovery (LOR) in goats. The aim of this study was to evaluate the effect of two FSH protocols for ovarian stimulation in goats on the expression patte...