In Vivo Imaging of Embryonic Vascular Development Using Transgenic Zebrafish

• Published in: Developmental biology Vol. 248; no. 2; pp. 307 - 318
• Main Authors: Lawson, Nathan D., Weinstein, Brant M.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.08.2002
• Subjects: angiogenesis; Animals; Animals, Genetically Modified; Blood Vessels - embryology; Brain - blood supply; Brain - embryology; Danio rerio; fli1 gene; Freshwater; Genes, Reporter - genetics; Germ-Line Mutation - genetics; Green Fluorescent Proteins; Luminescent Proteins - genetics; Luminescent Proteins - metabolism; Microscopy, Confocal - methods; Promoter Regions, Genetic - genetics; Time Factors; Transgenes - genetics; transgenic fish; transgenics; vascular development; zebrafish; Zebrafish - embryology; Zebrafish - genetics; Zebrafish Proteins - genetics; Zebrafish Proteins - metabolism; vascular development; zebrafish; angiogenesis; transgenics
• ISSN: 0012-1606; 1095-564X
• DOI: 10.1006/dbio.2002.0711

In this study we describe a model system that allows continuous in vivo observation of the vertebrate embryonic vasculature. We find that the zebrafish fli1 promoter is able to drive expression of enhanced green fluorescent protein (EGFP) in all blood vessels throughout embryogenesis. We demonstrate...