Enhanced sterol-acyl transferase activity promotes sterol accumulation in Saccharomyces cerevisiae

• Published in: Applied microbiology and biotechnology Vol. 53; no. 1; pp. 30 - 35
• Main Authors: Polakowski, T, Bastl, R, Stahl, U, Lang, C
• Format: Journal Article
• Language: English
• Published: Germany Springer Nature B.V 01.12.1999
• Subjects: Acyltransferases - metabolism; ARE2 gene; Deregulation; ergosterol; esterification; genes; HMG1 gene; Hydroxymethylglutaryl CoA Reductases - metabolism; hydroxymethylglutaryl-CoA reductases; lanosterol; Saccharomyces cerevisiae; Saccharomyces cerevisiae - metabolism; sterol-acyl transferase; Sterols; Sterols - metabolism; transcription (genetics); Yeast; Yeasts

The sterol-acyl transferase encoded by the gene ARE2 was transcriptionally deregulated in the yeast Saccharomyces cerevisiae to understand its role in sterol storage and sterol enrichment. Our results show that sterols can indeed be enriched in yeast by enhancing the capacity of the cells to esterif...