Inhibition of the PLP-dependent enzyme serine palmitoyltransferase by cycloserine: evidence for a novel decarboxylative mechanism of inactivation

• Published in: Molecular bioSystems Vol. 6; no. 9; pp. 1682 - 1693
• Main Authors: Lowther, Jonathan, Yard, Beverley A, Johnson, Kenneth A, Carter, Lester G, Bhat, Venugopal T, Raman, Marine C C, Clarke, David J, Ramakers, Britta, McMahon, Stephen A, Naismith, James H, Campopiano, Dominic J
• Format: Journal Article
• Language: English
• Published: England 01.01.2010
• Subjects: Chromatography, Liquid; Cycloserine - chemistry; Cycloserine - pharmacology; Enzyme Inhibitors - chemistry; Enzyme Inhibitors - pharmacology; Mass Spectrometry; Molecular Structure; Protein Structure, Secondary; Serine C-Palmitoyltransferase - antagonists & inhibitors; Serine C-Palmitoyltransferase - chemistry; Serine C-Palmitoyltransferase - genetics; Serine C-Palmitoyltransferase - metabolism; Sphingomonas - enzymology

Cycloserine (CS, 4-amino-3-isoxazolidone) is a cyclic amino acid mimic that is known to inhibit many essential pyridoxal 5'-phosphate (PLP)-dependent enzymes. Two CS enantiomers are known; D-cycloserine (DCS, also known as Seromycin) is a natural product that is used to treat resistant Mycobact...