Rheological properties of cryptococcal polysaccharide change with fiber size, antibody binding and temperature

is the major agent of cryptococcosis. The main virulence factor is the polysaccharide (PS) capsule. Changes in cryptococcal PS properties have been poorly elucidated. We analyzed the mechanical properties of secreted PS and intact capsules, using dynamic light scattering and optical tweezers. Storag...

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Bibliographic Details
Published inFuture microbiology Vol. 14; no. 10; pp. 867 - 884
Main Authors de S Araújo, Glauber R, Viana, Nathan B, Pontes, Bruno, Frases, Susana
Format Journal Article
LanguageEnglish
Published England Future Medicine Ltd 01.07.2019
Subjects
Antibodies, Fungal - immunology
Antibodies, Fungal - metabolism
capsule
Cryptococcosis
Cryptococcus neoformans - chemistry
Food
Fungal Capsules - chemistry
Fungal Capsules - immunology
Fungal Capsules - physiology
Fungal infections
fungal virulence
Infections
Light scattering
Mechanical properties
Microscopy
NMR
Nuclear magnetic resonance
Optical Tweezers
Particle Size
Polysaccharides
Polysaccharides - chemistry
Polysaccharides - immunology
Polysaccharides - metabolism
Polysaccharides - physiology
Rheology
secreted polysaccharide
Seeds
Temperature
Virulence factors
Virulence Factors - chemistry
Virulence Factors - immunology
Virulence Factors - metabolism
Virulence Factors - physiology
viscoelastic behavior
Viscoelastic Substances
Viscoelasticity
capsule
rheology
fungal virulence
viscoelastic behavior
secreted polysaccharide
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Summary:is the major agent of cryptococcosis. The main virulence factor is the polysaccharide (PS) capsule. Changes in cryptococcal PS properties have been poorly elucidated. We analyzed the mechanical properties of secreted PS and intact capsules, using dynamic light scattering and optical tweezers. Storage and loss moduli showed that secreted PS behaves as a viscoelastic liquid, while capsular PS behaves as a viscoelastic solid. The secreted PS remains as a viscoelastic fluid at different temperatures with thermal hysteresis after 85°C. Antibody binding altered the viscoelastic behavior of both secreted and capsular PS. Deciphering the mechanical aspects of these structures could reveal features that may have consequences in novel therapies against cryptococcosis.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:1746-0913
1746-0921
DOI:10.2217/fmb-2018-0320